Bračič Matej, Nagy Bence M, Plohl Olivija, Lackner Florian, Steindorfer Tobias, Fischer Roland C, Heinze Thomas, Olschewski Andrea, Kleinschek Karin Stana, Nagaraj Chandran, Mohan Tamilselvan
University of Maribor, Faculty of Mechanical Engineering, Laboratory for Characterisation and Processing of Polymers, Smetanova ulica17, 2000 Maribor, Slovenia.
Ludwig Boltzmann Institute for Lung Vascular Research, Stiftingtalstrasse 24, 8010 Graz, Austria.
iScience. 2024 Aug 12;27(9):110692. doi: 10.1016/j.isci.2024.110692. eCollection 2024 Sep 20.
Polyester biomaterials play a crucial in vascular surgery, but suffer from unspecific protein adsorption, thrombogenicity, and inadequate endothelial cell response, which limit their success. To address these issues, we investigated the functionalization of polyester biomaterials with antithrombogenic polysaccharide coatings. A two-step and water-based method was used to coat cationized polycaprolactone with different sulfated polysaccharides (SPS), which resulted in long-term stability, tunable morphology, roughness, film thickness, chemical compositions, zeta potential, and water content. The coatings significantly increased the anticoagulant activity and reduced the thrombogenicity of polycaprolactone, particularly with highly sulfated heparin and cellulose sulfate. Less SPS, such as chondroitin sulfate, fucoidan, and carrageenan, despite showing reduced anticoagulant activity, also exhibited lower fibrinogen adsorption. The adhesion and viability of human primary endothelial cells cultured on modified polycaprolactone correlated with the type and sulfate content of the coatings.
聚酯生物材料在血管外科手术中发挥着关键作用，但存在非特异性蛋白质吸附、血栓形成性和内皮细胞反应不足等问题，这些问题限制了它们的应用成效。为解决这些问题，我们研究了用抗血栓多糖涂层对聚酯生物材料进行功能化处理。采用两步法且基于水的方法，用不同的硫酸化多糖（SPS）包覆阳离子化聚己内酯，这产生了长期稳定性、可调节的形态、粗糙度、膜厚度、化学成分、zeta电位和含水量。这些涂层显著提高了聚己内酯的抗凝活性并降低了其血栓形成性，特别是对于高度硫酸化的肝素和硫酸纤维素而言。较少的SPS，如硫酸软骨素、岩藻依聚糖和角叉菜胶，尽管抗凝活性降低，但也表现出较低的纤维蛋白原吸附。在改性聚己内酯上培养的人原代内皮细胞的粘附和活力与涂层的类型和硫酸盐含量相关。
