Krauss-Etschmann Susanne, Hartl Dominik, Heinrich Joachim, Thaqi Agim, Prell Christine, Campoy Christina, Molina Francesco S, Hector Andreas, Decsi Tamas, Schendel Dolores J, Koletzko Berthold V
Childrens' Hospital of the Ludwig-Maximilians-University, Lindwurmstr. 2a, D-80337 Munich, Germany.
Clin Immunol. 2006 Feb-Mar;118(2-3):292-9. doi: 10.1016/j.clim.2005.10.003. Epub 2005 Nov 14.
The microbial environment in early infancy or even in utero may modulate the risk to develop allergic disease. Since Toll-like receptors (TLR) recognize microbial products, we hypothesized that maternal allergies may be associated with decreased levels of TLR2, TLR4 and CD14 mRNA in mothers and their offspring. 185 healthy pregnant women from Germany (n = 48), Hungary (n = 50) and Spain (n = 87) were enrolled in a European multicenter study. Levels of TLR2, TLR4 and CD14 mRNA were quantified in maternal peripheral blood samples taken at delivery and placental cord blood samples. Numbers of TLR2+, TLR4+ and CD14+ monocytes were quantified by flow cytometry in 42 cord blood samples obtained from the German participants. Bivariate and multivariate regression analyses were performed. Maternal allergies were associated with significantly lower levels of TLR2/4/CD14 mRNA in maternal blood and cord blood samples. Maternal and fetal TLR2/4/CD14 mRNA levels were significantly correlated with each other (TLR2 r = 0.42; TLR4 r = 0.58; CD14 r = 0.54). The results suggest that maternal allergy status may affect allergic risk in offspring through a decreased expression of fetal TLR2/4/CD14.
婴儿早期甚至子宫内的微生物环境可能会调节患过敏性疾病的风险。由于Toll样受体（TLR）可识别微生物产物，我们推测母亲的过敏反应可能与母亲及其后代体内TLR2、TLR4和CD14 mRNA水平降低有关。来自德国（n = 48）、匈牙利（n = 50）和西班牙（n = 87）的185名健康孕妇参与了一项欧洲多中心研究。在分娩时采集的母亲外周血样本和胎盘脐带血样本中对TLR2、TLR4和CD14 mRNA水平进行定量。通过流式细胞术对从德国参与者处获得的42份脐带血样本中的TLR2 +、TLR4 +和CD14 +单核细胞数量进行定量。进行了双变量和多变量回归分析。母亲的过敏反应与母亲血液和脐带血样本中TLR2/4/CD14 mRNA水平显著降低有关。母亲和胎儿的TLR2/4/CD14 mRNA水平彼此显著相关（TLR2 r = 0.42；TLR4 r = 0.58；CD14 r = 0.54）。结果表明，母亲的过敏状态可能通过降低胎儿TLR2/4/CD14的表达来影响后代的过敏风险。
