Shen Chung-Min, Lin Shih-Chang, Niu Dau-Ming, Kou Yu Ru
Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
Acta Paediatr. 2009 Jun;98(6):959-62. doi: 10.1111/j.1651-2227.2009.01280.x. Epub 2009 Apr 14.
Mode of delivery may influence the innate immune system in newborns. We investigated the effect of maternal labour on the expression of two Toll-like receptors, TLR2 and TLR4, in monocytes obtained from healthy full-term newborns.
Monocytes were obtained from cord blood of 48 newborns that have been vaginally delivered (VD) and 14 newborns delivered by elective caesarean section (CS) without labour. Peripheral blood was also obtained from 17 healthy adults. Surface expression of TLR2 and TLR4 in the monocytes was measured by antihuman TLR2 or TLR4 monoclonal antibody and immunofluorescence flow cytometry. TLR2 and TLR4 mRNA levels were evaluated by real-time PCR.
CS newborns had a significantly lower level of TLR2 and TLR4 surface expression on monocytes than VD newborns. No significant difference was found between the surface expression of VD newborns and healthy adults. TLR2 and TLR4 mRNA levels in monocytes did not vary among the three study groups.
Labour may up-regulate TLR2 and TLR4 on the cord blood monocytes of newborns at the protein level. Since TLRs are an important part of the innate immune system, our findings suggest that labour may be immunologically beneficial to normal newborns.
分娩方式可能会影响新生儿的先天免疫系统。我们研究了母体分娩对从健康足月新生儿获取的单核细胞中两种Toll样受体TLR2和TLR4表达的影响。
从48例经阴道分娩(VD)的新生儿和14例未经分娩而行择期剖宫产(CS)的新生儿的脐带血中获取单核细胞。还从17名健康成年人中获取外周血。通过抗人TLR2或TLR4单克隆抗体和免疫荧光流式细胞术测量单核细胞中TLR2和TLR4的表面表达。通过实时PCR评估TLR2和TLR4 mRNA水平。
CS新生儿单核细胞上TLR2和TLR4的表面表达水平明显低于VD新生儿。VD新生儿与健康成年人的表面表达之间未发现显著差异。三个研究组之间单核细胞中TLR2和TLR4 mRNA水平没有变化。
分娩可能会在蛋白质水平上上调新生儿脐带血单核细胞上的TLR2和TLR4。由于Toll样受体是先天免疫系统的重要组成部分,我们的研究结果表明分娩可能对正常新生儿具有免疫益处。