Kang Kyounglan, Oh Yeo Kyoung, Choue Ryowon, Kang Shin Jung
Research Institute of Clinical Nutrition, Kyung Hee University, Seoul, Korea.
Brain Res Mol Brain Res. 2005 Dec 14;142(2):139-45. doi: 10.1016/j.molbrainres.2005.09.006. Epub 2005 Nov 14.
Scutellariae radix is a Chinese herbal medicine that has been used to treat disease conditions accompanying inflammation and oxidative stress. In the present study, we examined the effect of Scutellariae radix extracts during acute ethanol exposure in N(2)a neuroblastoma. The Scutellariae radix extracts effectively inhibited ethanol-induced apoptosis and caspase-3/-7 activation. Ethanol induced the expression of caspase-11 that has been known as a dual regulator of pathological apoptosis and inflammatory response. The ethanol-induced caspase-11 expression was suppressed by pretreatment of the Scutellariae radix extracts. Furthermore, the activation of caspase-3/-7 and apoptosis were significantly inhibited in caspase-11-/- mouse embryonic fibroblasts following ethanol treatment. These results suggest that caspase-11 has a regulatory role in ethanol-induced apoptosis, and the suppression of caspase-11 may be a mechanism by which Scutellariae radix exerts its cytoprotective effect.
黄芩是一种用于治疗伴有炎症和氧化应激疾病的中草药。在本研究中，我们检测了黄芩提取物在N(2)a神经母细胞瘤急性乙醇暴露期间的作用。黄芩提取物有效抑制了乙醇诱导的细胞凋亡和半胱天冬酶-3/-7的激活。乙醇诱导了半胱天冬酶-11的表达，该蛋白已知是病理性细胞凋亡和炎症反应的双重调节因子。乙醇诱导的半胱天冬酶-11表达被黄芩提取物预处理所抑制。此外，乙醇处理后，在半胱天冬酶-11基因敲除小鼠胚胎成纤维细胞中，半胱天冬酶-3/-7的激活和细胞凋亡受到显著抑制。这些结果表明，半胱天冬酶-11在乙醇诱导的细胞凋亡中具有调节作用，而对半胱天冬酶-11的抑制可能是黄芩发挥其细胞保护作用的机制。
