Das Shraboni, Jain Tapan Kumar, Maitra Amarnath
Department of Chemistry, University of Delhi, Delhi, 110007, India.
J Colloid Interface Sci. 2002 Aug 1;252(1):82-8. doi: 10.1006/jcis.2002.8404.
Ormosil (organically modified silane) such as n-octyl triethoxy silane has been found to aggregate in the form of normal micelles as well as reverse micelles in which the triethoxy silane moeities are hydrolyzed to form a hydrated silica network while the n-octyl groups are held together through hydrophobic interaction. These nanoparticles are spherical in shape and are nearly monodispersed with an average diameter of below 100 nm. The nanoparticles originating from the micellar aggregate have an hydrophobic core with a layer of the hydrated silica network at the surface. The hydrophobic core can host hydrophobic molecules such as tetraphenyl porphyrin, which is leached out of the particles extremely slowly compared to that in Triton X-100 micelles. The nanoparticles originating from the reverse micelles have a hydrated silica network in the core surrounded by the hydrophobic n-octyl chains on the particle surface. The hydrophilic silica cores of these nanoparticles have been used to encapsulate horseradish peroxidase (HRP) and the enzyme shows its activity and follows Michaelis-Menten kinetics.
已发现诸如正辛基三乙氧基硅烷之类的有机改性硅烷（Ormosil）会以正常胶束以及反胶束的形式聚集，其中三乙氧基硅烷基团水解形成水合二氧化硅网络，而正辛基基团则通过疏水相互作用聚集在一起。这些纳米颗粒呈球形，几乎单分散，平均直径低于100纳米。源自胶束聚集体的纳米颗粒具有疏水核心，表面有一层水合二氧化硅网络。疏水核心可以容纳诸如四苯基卟啉之类的疏水分子，与在Triton X - 100胶束中的情况相比，该分子从颗粒中渗出的速度极慢。源自反胶束的纳米颗粒在核心处有一个水合二氧化硅网络，颗粒表面被疏水的正辛基链包围。这些纳米颗粒的亲水性二氧化硅核心已被用于包封辣根过氧化物酶（HRP），并且该酶表现出其活性并遵循米氏动力学。
