Santra Swadeshmukul, Dutta Debamitra, Walter Glenn A, Moudgil Brij M
Nanoscience Technology Center, Department of Chemistry and Biomolecular Science Center, University of Central Florida, Research Pavilion, Orlando, 32826, USA.
Technol Cancer Res Treat. 2005 Dec;4(6):593-602. doi: 10.1177/153303460500400603.
Optical imaging technique has strong potential for sensitive cancer diagnosis, particularly at the early stage of cancer development. This is a sensitive, non-invasive, non-ionizing (clinically safe) and relatively inexpensive technique. Cancer imaging with optical technique however greatly relies upon the use of sensitive and stable optical probes. Unlike the traditional organic fluorescent probes, fluorescent nanoparticle probes such as dye-doped nanoparticles and quantum dots (Qdots) are bright and photostable. Fluorescent nanoparticle probes are shown to be very effective for sensitive cancer imaging with greater success in the cellular level. However, cancer imaging in an in vivo setup has been recently realized. There are several challenges in developing fluorescent nanoparticle probes for in vivo cancer imaging applications. In this review, we will discuss various aspects of nanoparticle design, synthesis, surface functionalization for bioconjugation and cancer cell targeting. A brief overview of in vivo cancer imaging with Qdots will also be presented.
光学成像技术在敏感的癌症诊断方面具有强大潜力，尤其是在癌症发展的早期阶段。这是一种敏感、非侵入性、非电离（临床安全）且相对廉价的技术。然而，光学技术用于癌症成像在很大程度上依赖于使用灵敏且稳定的光学探针。与传统的有机荧光探针不同，诸如染料掺杂纳米颗粒和量子点（Qdots）之类的荧光纳米颗粒探针亮度高且光稳定性好。荧光纳米颗粒探针已被证明在细胞水平上对敏感的癌症成像非常有效，并且取得了更大的成功。然而，最近才实现了体内环境下的癌症成像。在开发用于体内癌症成像应用的荧光纳米颗粒探针方面存在若干挑战。在这篇综述中，我们将讨论纳米颗粒设计、合成、用于生物共轭和癌细胞靶向的表面功能化的各个方面。还将简要概述使用量子点进行的体内癌症成像。
