Kirtzel Julia, Madhavan Soumya, Wielsch Natalie, Blinne Alexander, Hupfer Yvonne, Linde Jörg, Krause Katrin, Svatoš Aleš, Kothe Erika
Microbial Communication, Institute of Microbiology, Friedrich Schiller University, Jena, Germany.
Research Group Mass Spectrometry/Proteomics, Max Planck Institute for Chemical Ecology, Jena, Germany.
Front Microbiol. 2018 Oct 24;9:2545. doi: 10.3389/fmicb.2018.02545. eCollection 2018.
is a filamentous basidiomycete causing white-rot in many wood species with the help of a broad range of enzymes including multicopper oxidases such as laccases and laccase-like oxidases. Since these enzymes exhibit a broad substrate range, their ability to oxidatively degrade black slate was investigated. Both haploid monokaryotic, and mated dikaryotic strains were able to grow on black slate rich in organic carbon as sole carbon source. On defined media, only the monokaryon showed growth promotion by addition of slate. At the same time, metals were released from the slate and, after reaching a threshold concentration, inhibited further growth of the fungus. The proteome during decomposition of the black slate showed induction of proteins potentially involved in rock degradation and stress resistance, and the gene for laccase-like oxidase was up-regulated. Specifically in the dikaryon, the laccase gene was induced, while as well as , and expression levels remained similar. Spectrophotometric analysis revealed that both life forms were able to degrade the rock and produce smaller particles.
它是一种丝状担子菌，借助包括漆酶等多铜氧化酶和漆酶样氧化酶在内的多种酶，能在许多木材种类中引发白腐病。由于这些酶具有广泛的底物范围，因此对它们氧化降解黑色板岩的能力进行了研究。单倍体单核菌株和配对后的双核菌株都能够在富含有机碳的黑色板岩作为唯一碳源的培养基上生长。在特定培养基上，只有单核体在添加板岩后显示出生长促进作用。与此同时，金属从板岩中释放出来，在达到阈值浓度后，会抑制真菌的进一步生长。黑色板岩分解过程中的蛋白质组显示，可能参与岩石降解和抗逆性的蛋白质被诱导表达，漆酶样氧化酶基因上调。具体而言，在双核体中，漆酶基因被诱导，而 以及 和 的表达水平保持相似。分光光度分析表明，两种生命形式都能够降解岩石并产生更小的颗粒。
