Reddy G R, Dame J B
Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville 32611.
J Clin Microbiol. 1992 Jul;30(7):1811-4. doi: 10.1128/jcm.30.7.1811-1814.1992.
Three synthetic oligonucleotide probes complementary to unique regions of Babesia bigemina small-subunit rRNA were developed for detecting the parasite in bovine blood. These probes specifically detected a parasitemia of 2 x 10(-5)% by autoradiography in less than 24 h by using a 200-microliters sample of bovine blood. These probes did not bind to total RNA or genomic DNA isolated from another closely related species, Babesia bovis, or to bovine leukocyte RNA. This method detected B. bigemina infections in calves inoculated with as few as 1,000 infected erythrocytes from the second day onward for 16 days.
开发了三种与双芽巴贝斯虫小亚基rRNA独特区域互补的合成寡核苷酸探针，用于检测牛血液中的寄生虫。通过放射自显影，使用200微升牛血样本，这些探针在不到24小时内就能特异性检测到2×10⁻⁵%的寄生虫血症。这些探针不与从另一个密切相关的物种——牛巴贝斯虫分离的总RNA或基因组DNA结合，也不与牛白细胞RNA结合。该方法从第二天起，在接种了低至1000个感染红细胞的小牛中检测双芽巴贝斯虫感染，持续16天。
