Lal A A, Changkasiri S, Hollingdale M R, McCutchan T F
Malaria Department, Biomedical Research Institute, Rockville, MD.
Mol Biochem Parasitol. 1989 Aug;36(1):67-71. doi: 10.1016/0166-6851(89)90201-6.
We have identified useful target sites for the diagnosis of malaria infections by oligonucleotide hybridization on the small subunit RNA of Plasmodium falciparum. Acetic acid works as effectively as formaldehyde or methyl mercuric hydroxide in procedures designed to apply RNA to filters. We have confirmed the findings of others that the stability of ribosomal RNA suffices for its use as a target for diagnosis. We have achieved a detection level of at least 0.00046% parasitemia and suggest that detection of a single parasite is well within reach of this technology.
我们已经在恶性疟原虫的小亚基RNA上确定了通过寡核苷酸杂交诊断疟疾感染的有用靶位点。在将RNA应用于滤膜的程序中，乙酸的效果与甲醛或氢氧化甲基汞一样有效。我们已经证实了其他人的发现，即核糖体RNA的稳定性足以使其用作诊断靶标。我们已经实现了至少0.00046%的寄生虫血症检测水平，并表明检测单个寄生虫在这项技术的能力范围内。
