Vega C A, Buening G M, Rodriguez S D, Carson C A
J Protozool. 1986 Nov;33(4):514-8. doi: 10.1111/j.1550-7408.1986.tb05653.x.
Clones of in vitro-cultured Babesia bigemina-infected erythrocytes were concentrated by several density gradient procedures. The density range of infected erythrocytes containing pairs of parasites was 1.077 to 1.089 g/ml, whereas the density range of infected erythrocytes containing single parasites was 1.092 to 1.100 g/ml. Three enzymes--lactate dehydrogenase, glucose-phosphate isomerase, and glutamate dehydrogenase--were found associated with infected erythrocytes. The parasite-specific enzyme and/or isoenzymes were shown to have different mobility patterns in starch gel electrophoresis from those found in the normal bovine erythrocytes. The enzyme 6-phosphogluconate dehydrogenase was not detected as a parasite-specific enzyme in B. bigemina-infected erythrocytes.
通过几种密度梯度程序对体外培养的感染双芽巴贝斯虫的红细胞克隆进行浓缩。含有成对寄生虫的感染红细胞的密度范围为1.077至1.089克/毫升，而含有单个寄生虫的感染红细胞的密度范围为1.092至1.100克/毫升。发现三种酶——乳酸脱氢酶、葡萄糖磷酸异构酶和谷氨酸脱氢酶——与感染红细胞相关。在淀粉凝胶电泳中，寄生虫特异性酶和/或同工酶显示出与正常牛红细胞中发现的酶不同的迁移模式。在感染双芽巴贝斯虫的红细胞中未检测到6-磷酸葡萄糖酸脱氢酶作为寄生虫特异性酶。
