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Distribution of heartwater in the Caribbean determined on the basis of detection of antibodies to the conserved 32-kilodalton protein of Cowdria ruminantium.

作者信息

Muller Kubold A, Martinez D, Camus E, Jongejan F

机构信息

Department of Parasitology and Tropical Veterinary Medicine, Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht, The Netherlands.

出版信息

J Clin Microbiol. 1992 Jul;30(7):1870-3. doi: 10.1128/jcm.30.7.1870-1873.1992.

DOI:10.1128/jcm.30.7.1870-1873.1992
PMID:1629346
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265396/
Abstract

A competitive enzyme-linked immunosorbent assay (cELISA) was developed to detect immunoglobulin G antibodies to the major 32-kDa protein of Cowdria ruminantium. A total of 1,804 serum samples collected from cattle on 19 islands in the eastern Caribbean Basin were tested by this cELISA. A total of 133 serum samples from 10 islands (Antigua, Dominica, Grenada, Guadeloupe, Martinique, Montserrat, St. Kitts, St. Lucia, St. Martin, and St. Vincent) were found to be positive. The presence of antibodies to C. ruminantium in cattle on these islands was confirmed by immunofluorescence and Western blotting (immunoblotting). In earlier studies, C. ruminantium has been demonstrated only on Guadeloupe, Antigua, and Marie Galante. This study shows that the causative agent of heartwater is now firmly established in the Caribbean.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e184/265396/0b1b799e28ad/jcm00031-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e184/265396/0b1b799e28ad/jcm00031-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e184/265396/0b1b799e28ad/jcm00031-0270-a.jpg

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本文引用的文献

1
[Cowdriosis (Heartwater) by Cowdria ruminantium in ruminants of French Indies (Guadeloupe) and Mascarene Islands (La Reunion and Mauritius)].[法属安的列斯群岛（瓜德罗普岛）和马斯克林群岛（留尼汪岛和毛里求斯）反刍动物中由反刍动物考德里氏体引起的考德里氏体病（心水病）]
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Heartwater in the Caribbean: isolation of Cowdria ruminantium from Antigua.
一种基于反刍动物考德里氏体免疫显性32千道尔顿蛋白的牛心水病免疫印迹诊断检测方法在现场血清中检测到假阳性。
J Clin Microbiol. 1993 Oct;31(10):2729-37. doi: 10.1128/jcm.31.10.2729-2737.1993.
4
Production of alpha interferon in Cowdria ruminantium-infected cattle and its effect on infected endothelial cell cultures.感染反刍兽考德里氏体的牛体内α干扰素的产生及其对受感染内皮细胞培养物的影响。
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Correlation between antibodies to Cowdria ruminantium (rickettsiales) in cattle and the distribution of Amblyomma vector ticks in Zimbabwe.津巴布韦牛体内反刍动物考德里氏体（立克次氏体目）抗体与钝缘蜱传播媒介蜱分布的相关性
Exp Appl Acarol. 1993 Nov;17(11):799-810. doi: 10.1007/BF00225853.
6
Use of a specific immunogenic region on the Cowdria ruminantium MAP1 protein in a serological assay.在血清学检测中使用反刍动物考德里氏体MAP1蛋白上的特定免疫原性区域。
J Clin Microbiol. 1995 Sep;33(9):2405-10. doi: 10.1128/jcm.33.9.2405-2410.1995.
7
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Exp Appl Acarol. 1992 Aug;15(2):117-21. doi: 10.1007/BF01275522.
Vet Rec. 1985 Feb 2;116(5):121-3. doi: 10.1136/vr.116.5.121.
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[A strain of Cowdria ruminantium isolated in Guadeloupe (French West Indies)].
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Identification of an immunodominant antigenically conserved 32-kilodalton protein from Cowdria ruminantium.从反刍动物考德里氏体中鉴定出一种免疫显性的、抗原性保守的32千道尔顿蛋白。
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