Bansal Seema, Jain Amita, Agarwal Jyotsna, Malik G K
Post Graduate Department of Microbiology, King George's Medical University, Lucknow.
Indian J Pathol Microbiol. 2004 Oct;47(4):586-8.
Present study was undertaken for establishing significance of coagulase negative staphylococci isolated from cases of late onset neonatal septicemia. 660 neonates admitted to NICU with clinical suspicion of late onset septicemia, over a period of nine months, were included in study. After skin preparation 1.5-ml blood for culture was collected from two different sites by venipuncture and each was inoculated into a blood culture bottle. All CONS thus isolated were further analysed. Laboratory criteria for significant CONS bacteremia was defined as recovery of CONS with in 48 hours of specimen collection from both sites of a blood culture set that displayed uniform antibiotic susceptibility and biochemical reactions. Due to technical difficulties two samples for blood culture were obtained only from 338 cases, CONS were recovered from 52 (22.7%) cases; only 13 (25%) were considered significant. Only single blood sample was available from remaining 322 subjects and CONS were recovered from 36/322 (24.3%). CONS isolation rate was similar in both subject groups. Using double specimen protocol we found majority of CONS recovered from neonates, to be probable contaminants. Recovery of CONS from blood of a septicemic neonate needs to be viewed with caution since not all of them are true bacteremic agents.
本研究旨在确定从晚发性新生儿败血症病例中分离出的凝固酶阴性葡萄球菌的意义。在九个月的时间里，660名因临床怀疑患有晚发性败血症而入住新生儿重症监护病房的新生儿被纳入研究。在皮肤准备后，通过静脉穿刺从两个不同部位采集1.5毫升用于培养的血液，并将每份血液接种到一个血培养瓶中。对所有如此分离出的凝固酶阴性葡萄球菌进行进一步分析。显著凝固酶阴性葡萄球菌菌血症的实验室标准定义为，在从血培养套装的两个部位采集标本后的48小时内，分离出显示出一致的抗生素敏感性和生化反应的凝固酶阴性葡萄球菌。由于技术困难，仅从338例病例中获得了两份血培养样本，从52例（22.7%）病例中分离出了凝固酶阴性葡萄球菌；只有13例（25%）被认为是显著的。其余322名受试者仅获得了一份血样，从36/322（24.3%）中分离出了凝固酶阴性葡萄球菌。两个受试者组中的凝固酶阴性葡萄球菌分离率相似。使用双样本方案，我们发现从新生儿中分离出的大多数凝固酶阴性葡萄球菌可能是污染物。对于败血症新生儿血液中凝固酶阴性葡萄球菌的分离结果需要谨慎看待，因为并非所有的都是真正的菌血症病原体。
