Flieger M, Kantorová M, Halada P, Kuzma M, Pazoutová S, Stodůlková E, Kolínská R
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czechia.
Folia Microbiol (Praha). 2005;50(3):198-204. doi: 10.1007/BF02931566.
Oligosaccharides produced by submerged cultures of C. africana and C. sorghi were isolated by semipreparative HPLC. Structure of 6-O-beta-D-fructofuranosyl-D-glucopyranose (blastose), 1,6-bis-O-(beta-D-fructofuranosyl)-alpha-D-glucopyranoside (neokestose) and two sugar alcohols, 1-O-beta-D-fructofuranosyl-D-mannitol (fructosylmannitol) and 1,6-bis-O-(beta-D-fructofuranosyl)-D-mannitol (bisfructosylmannitol) was determined by NMR spectrometry. MALDI TOF MS analysis revealed molecular ions [M+Na]+ that indicate the presence of other tetra- and pentasaccharides (m/z = 689.4 and 851.5, respectively) and corresponding sugar alcohol (m/z = 691.4). Rapid conversion of sucrose into series of oligosaccharides and corresponding sugar alcohols was observed in all tested strains.
通过半制备高效液相色谱法分离了非洲炭疽菌和高粱炭疽菌深层培养产生的寡糖。通过核磁共振光谱法确定了6-O-β-D-呋喃果糖基-D-吡喃葡萄糖（蔗果三糖）、1,6-双-O-（β-D-呋喃果糖基）-α-D-吡喃葡萄糖苷（新蔗果四糖）以及两种糖醇，1-O-β-D-呋喃果糖基-D-甘露醇（果糖基甘露醇）和1,6-双-O-（β-D-呋喃果糖基）-D-甘露醇（双果糖基甘露醇）的结构。基质辅助激光解吸电离飞行时间质谱分析揭示了分子离子[M+Na]+，表明存在其他四糖和五糖（分别为m/z = 689.4和851.5）以及相应的糖醇（m/z = 691.4）。在所有测试菌株中均观察到蔗糖快速转化为一系列寡糖和相应的糖醇。
