Mahmood N, Hay A J
MRC Collaborative Centre, Mill Hill, London, UK.
J Immunol Methods. 1992 Jul 6;151(1-2):9-13. doi: 10.1016/0022-1759(92)90101-x.
A simple, reliable ELISA for the quantitative detection of the envelope glycoproteins of both HIV and SIV is described. It incorporates the snowdrop lectin GNA to capture the glycoprotein antigens and combines the high selectivity of GNA binding with its broad reactivity with the glycoproteins of HIV-1, HIV-2 and SIV.
本文描述了一种用于定量检测HIV和SIV包膜糖蛋白的简单、可靠的酶联免疫吸附测定(ELISA)。它采用雪花莲凝集素GNA来捕获糖蛋白抗原,并将GNA结合的高选择性与其对HIV-1、HIV-2和SIV糖蛋白的广泛反应性相结合。