Gilljam G
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
AIDS Res Hum Retroviruses. 1993 May;9(5):431-8. doi: 10.1089/aid.1993.9.431.
Lectin affinity chromatography was used to purify in a single step the envelope glycoproteins of HIV-1, HIV-2, and SIV. Envelope glycoproteins carry the major determinants essential for protection by the humoral immune response. The purification of these proteins has previously been a laborious procedure. The glycoproteins were purified by a one-step procedure to a high level of purity by using Galanthus nivalis agglutinin (GNA). The purified glycoprotein had CD4-binding and antigenic reactivities. Strong immune responses to envelope proteins and peptides were seen in mice and primates after immunization with these preparations.
凝集素亲和层析法被用于一步纯化HIV-1、HIV-2和SIV的包膜糖蛋白。包膜糖蛋白携带体液免疫反应提供保护所必需的主要决定簇。这些蛋白质的纯化此前一直是个费力的过程。通过使用雪花莲凝集素（GNA），这些糖蛋白通过一步法被纯化到了高纯度水平。纯化后的糖蛋白具有CD4结合和抗原反应性。在用这些制剂免疫后，在小鼠和灵长类动物中观察到了对包膜蛋白和肽的强烈免疫反应。
