产气荚膜梭菌突变体的分离与鉴定，这些突变体在血凝素和唾液酸酶产生方面均发生改变。
Isolation and characterization of Clostridium perfringens mutants altered in both hemagglutinin and sialidase production.
作者信息
Rood J I, Wilkinson R G
出版信息
J Bacteriol. 1975 Aug;123(2):419-27. doi: 10.1128/jb.123.2.419-427.1975.
Abstract
The relationship between the production of hemagglutinin and sialidase activities by Clostridium perfringens was investigated by screening for mutants producing reduced levels of hemagglutinin activity. Twelve mutants were isolated; all produced reduced levels of sialidase activity and several had other altered phenotypic markers. Revertants that regained the ability to produce active hemagglutinin were isolated. All of these revertants produced increased sialidase activity. These results show that the production of hemagglutinin activity is directly related to the production of sialidase activity. Evidence is also presented that the processes of sporulation and the production of extracellular proteins are interrelated.
摘要
通过筛选血凝素活性水平降低的突变体，研究了产气荚膜梭菌产生血凝素与唾液酸酶活性之间的关系。分离出12个突变体；所有突变体产生的唾液酸酶活性水平均降低，且有几个具有其他改变的表型标记。分离出恢复产生活性血凝素能力的回复突变体。所有这些回复突变体产生的唾液酸酶活性均增加。这些结果表明，血凝素活性的产生与唾液酸酶活性的产生直接相关。还提供了证据表明芽孢形成过程与细胞外蛋白质的产生是相互关联的。
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