在无蛋黄胰蛋白胨亚硫酸盐环丝氨酸琼脂中对粪便产气荚膜梭菌芽孢进行计数。
Enumeration of fecal Clostridium perfringens spores in egg yolk-free tryptose-sulfite-cycloserine agar.
作者信息
Hauschild A H, Hilsheimer R, Griffith D W
出版信息
Appl Microbiol. 1974 Mar;27(3):527-30. doi: 10.1128/am.27.3.527-530.1974.
Abstract
The Shahidi-Ferguson perfringens, tryptose-sulfite-cycloserine (TSC), and egg yolk-free TSC agars have been tested for their suitability to enumerate fecal spores of Clostridium perfringens. When these spores comprised at least 20% of the total anaerobe spores, equally accurate counts were obtained in the three media. With lower ratios of C. perfringens spores, the most accurate counts were obtained in egg yolk-free TSC agar. The median C. perfringens spore count of 60 normal fecal specimens was log 3.4/g. A nonmotile, sulfite- and nitrate-reducing Clostridium, not identifiable with any known clostridial species, was isolated from 14 out of 60 fecal specimans. It was not differentiated from C. perfringens in the nitrite motility test, but could be distinguished by its inability to liquefy gelatin.
摘要
已对沙希迪-弗格森产气荚膜梭菌、胰蛋白胨亚硫酸盐环丝氨酸（TSC）和无蛋黄TSC琼脂进行测试，以评估它们用于计数产气荚膜梭菌粪便孢子的适用性。当这些孢子至少占厌氧孢子总数的20%时，在这三种培养基中获得的计数同样准确。当产气荚膜梭菌孢子比例较低时，在无蛋黄TSC琼脂中获得的计数最准确。60份正常粪便标本中产气荚膜梭菌孢子计数的中位数为每克log 3.4。从60份粪便标本中的14份中分离出一种不能运动、能还原亚硫酸盐和硝酸盐的梭菌，无法将其鉴定为任何已知的梭菌属物种。在亚硝酸盐动力试验中，它与产气荚膜梭菌无法区分，但可通过其不能液化明胶来加以区分。
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