Juang J-H, Hsu B R-S, Kuo C-H
Division of Endocrinology and Metabolism, Department of Internal Medicine, Chang Gung Memorial Hospital, 5 Fu-Shin Street, Kweishan, Taoyuan, Taiwan.
Transplant Proc. 2005 Oct;37(8):3479-81. doi: 10.1016/j.transproceed.2005.09.023.
The subcutaneous site is ideal for clinical islet transplantation because it has the advantage of being accessible and can be biopsied when needed. Sadly, the results at subcutaneous sites were disappointing. The reason for this failure is not known, but poor vascularization may play a role. We tested the hypothesis that islet grafts would do better if more vasculature or oxygen could be supplied. Six hundred isolated C57BL/6 mouse islets were syngeneically transplanted into inbred streptozotocin-diabetic recipients at a subcutaneous site on the back with (Group A, n = 6) or without (Group B, n = 8) postoperative hyperbaric oxygen (2.4 ATA, 100% O(2)) therapy, or at a calf muscle (Group C, n = 9). During 13-week posttransplantation follow-up, recipients' blood glucose decreased and body weight increased significantly in all 3 groups (P < .05). However, there was no significant difference among the 3 groups. At 13 weeks, the insulin contents of the graft was also comparable among the 3 groups. Our data indicate the following: (1) postoperative hyperbaric oxygen therapy did not improve the outcome of islet transplantation at a subcutaneous site; and (2) a muscular site was not superior to a subcutaneous site for islet transplantation.
皮下部位是临床胰岛移植的理想部位，因为它具有易于操作的优势，并且在需要时可以进行活检。遗憾的是，皮下部位的移植结果并不理想。失败的原因尚不清楚，但血管化不良可能起了一定作用。我们检验了这样一个假设，即如果能够提供更多的血管或氧气，胰岛移植效果会更好。将600个分离的C57BL/6小鼠胰岛同基因移植到链脲佐菌素诱导的糖尿病近交系受体的背部皮下部位，其中一组（A组，n = 6）术后接受高压氧（2.4 ATA，100% O₂）治疗，另一组（B组，n = 8）不接受治疗，还有一组（C组，n = 9）移植到小腿肌肉。在移植后的13周随访期间，所有3组受体的血糖均显著下降，体重显著增加（P <.05）。然而，3组之间没有显著差异。在13周时，3组移植组织中的胰岛素含量也相当。我们的数据表明：（1）术后高压氧治疗并未改善皮下部位胰岛移植的结果；（2）对于胰岛移植而言，肌肉部位并不优于皮下部位。
