Nishizawa Tsutomu, Takahashi Masaharu, Endo Kazunori, Fujiwara Shinji, Sakuma Nobuo, Kawazuma Fumiaki, Sakamoto Hirotsugu, Sato Yukihiro, Bando Masashi, Okamoto Hiroaki
Division of Virology, Department of Infection and Immunity, Jichi Medical School, Tochigi-Ken 329-0498, Japan.
Koyadairamura Kokuho Clinic, Tokushima-Ken 777-0302, Japan.
J Gen Virol. 2005 Dec;86(Pt 12):3321-3326. doi: 10.1099/vir.0.81394-0.
Two (2.3 %) of 87 wild-caught boars in Japan had detectable hepatitis E virus (HEV) RNA. The two boar HEV isolates (wbJTS1 and wbJYG1) obtained in the present study and a previously reported isolate (wbJSG1) whose partial sequence had been determined were sequenced over the entire genome. The wbJSG1, wbJTS1 and wbJYG1 isolates comprised 7225 or 7226 nt, excluding the poly(A) tail, and segregated into genotype 3. They differed by 8.5-11.2 % from each other and by 8.6-18.4 % from 17 reported genotype 3 HEV isolates, including one boar isolate, in the full-length sequence. When compared with 191 reported genotype 3 HEV isolates whose partial sequences were known, these three boar isolates were closer to Japanese isolates than to isolates of non-Japanese origin (89.2+/-2.6 vs 85.9+/-2.2 %; P<0.0001). A proportion of wild boars in Japan are infected with markedly heterogeneous HEV strains that are indigenous to Japan and may serve as reservoirs of HEV.
在日本捕获的87头野猪中，有2头（2.3%）检测到戊型肝炎病毒（HEV）RNA。对本研究中获得的2株野猪HEV分离株（wbJTS1和wbJYG1）以及之前报道过的已测定部分序列的分离株（wbJSG1）进行了全基因组测序。wbJSG1、wbJTS1和wbJYG1分离株不包括聚腺苷酸尾，由7225或7226个核苷酸组成，属于3型基因型。它们彼此之间的差异为8.5%-11.2%，与17株已报道的3型基因型HEV分离株（包括1株野猪分离株）的全长序列差异为8.6%-18.4%。与191株已知部分序列的已报道3型基因型HEV分离株相比，这3株野猪分离株与日本分离株的亲缘关系比与非日本来源的分离株更近（89.2±2.6%对85.9±2.2%；P<0.0001）。日本的一部分野猪感染了明显异质的HEV毒株，这些毒株是日本本土的，可能是HEV的储存宿主。
