蓝靛果忍冬（蓝果忍冬）中花色苷和多酚的表征及定量分析

Characterization and quantification of anthocyanins and polyphenolics in bluehHoneysuckle (Lonicera caerulea L.).

作者信息

Chaovanalikit Arusa, Thompson Maxine M, Wrolstad Ronald E

机构信息

Department of Food Science and Technology, 100 Wiegand Hall, Oregon State University, Corvallis, Oregon 97331-6602, USA.

出版信息

J Agric Food Chem. 2004 Feb 25;52(4):848-52. doi: 10.1021/jf030509o.

DOI:10.1021/jf030509o

PMID:14969540

Abstract

Anthocyanins and phenolics of 10 blue honeysuckle (Lonicera caerulea L.) genotypes were characterized and quantified by HPLC-DAD. Peak assignments were confirmed by low-resolution electrospray mass spectrometry. Six anthocyanins were detected with the major peak identified as cyanidin 3-glucoside. Five additional anthocyanins were characterized as cyanidin 3,5-diglucoside, cyanidin 3-rutinoside, pelargonidin 3-glucoside, peonidin 3-glucoside, and peonidin 3-rutinoside. Four polyphenolics were identified as chlorogenic acid, neochlorogenic acid, quercetin 3-rutinoside, and quercetin 3-glucoside. Two additional unidentified phenolics were characterized as flavonol and hydroxycinnamic derivatives based on UV-vis spectra. Hydroxycinnamate levels ranged from 30.4 to 156.2 mg/100 g, whereas the flavonol content ranged from 12.6 to 32.8 mg/100 g. The L. caerulea subspecies boczkarnikovae contained the highest amounts of hydroxycinnamic derivatives and flavonols.

摘要

采用高效液相色谱-二极管阵列检测器（HPLC-DAD）对10种蓝靛果忍冬（Lonicera caerulea L.）基因型的花色苷和酚类物质进行了表征和定量分析。通过低分辨率电喷雾质谱对峰进行了确认。检测到6种花色素苷，主要峰被鉴定为矢车菊素3-葡萄糖苷。另外5种花色素苷被鉴定为矢车菊素3,5-二葡萄糖苷、矢车菊素3-芸香糖苷、天竺葵素3-葡萄糖苷、芍药素3-葡萄糖苷和芍药素3-芸香糖苷。4种多酚类物质被鉴定为绿原酸、新绿原酸、槲皮素3-芸香糖苷和槲皮素3-葡萄糖苷。基于紫外可见光谱，另外2种未鉴定的酚类物质被表征为黄酮醇和羟基肉桂酸衍生物。羟基肉桂酸含量在30.4至156.2毫克/100克之间，而黄酮醇含量在12.6至32.8毫克/100克之间。蓝靛果忍冬亚种博氏蓝靛果忍冬中羟基肉桂酸衍生物和黄酮醇的含量最高。