Rodríguez-Iñigo E, Bartolomé J, Ortiz-Movilla N, Platero C, López-Alcorocho J M, Pardo M, Castillo I, Carreño V
Fundación para el Estudio de las Hepatitis Virales, C/ Guzmán El Bueno, 72, 28015 Madrid, Spain.
J Virol. 2005 Dec;79(24):15578-81. doi: 10.1128/JVI.79.24.15578-15581.2005.
In this work, we have shown that hepatitis C virus (HCV) and hepatitis B virus (HBV) can coexist in the same hepatocyte using double fluorescent in situ hybridization in liver biopsy samples from patients with chronic HCV infection with occult HBV infection. Digital image analysis of hybridization signals showed that the HBV DNA levels in coinfected hepatocytes were lower than those in cells infected only with HBV. This finding supports the hypothesis of inhibition of HBV replication by HCV. Furthermore, HCV RNA levels were lower in coinfected cells than in cells infected only with HCV, suggesting that HBV may also inhibit HCV replication.
在这项研究中，我们利用双重荧光原位杂交技术，在慢性丙型肝炎病毒（HCV）感染合并隐匿性乙型肝炎病毒（HBV）感染患者的肝活检样本中，证实了HCV和HBV可共存于同一肝细胞中。杂交信号的数字图像分析显示，合并感染的肝细胞中HBV DNA水平低于仅感染HBV的细胞。这一发现支持了HCV抑制HBV复制的假说。此外，合并感染细胞中的HCV RNA水平低于仅感染HCV的细胞，提示HBV也可能抑制HCV复制。
