Ly Suw Young
Biosensor Research Institute in Seoul National University of Technology, 172, gongreung 2 dong Nowon gu, Seoul, South Korea, 139-743.
Bioelectrochemistry. 2006 May;68(2):227-31. doi: 10.1016/j.bioelechem.2005.09.002. Epub 2005 Nov 23.
A simply prepared DNA immobilized on a carbon nanotube paste electrode (CNTPE) was utilized to monitor dopamine ion concentration using the cyclic voltammetry (CV) and square-wave (SW) stripping voltammetry methods. The optimum analytical conditions were sought. The result obtained was a very low detection limit compared to other common voltammetry methods. The optimal parameters were found to be as follows: 3.5 pH, 0.48 V SW amplitude, 71 Hz frequency, 5 s accumulation time, 0.01 V increment potential, and -1.3 V (anodic-*-) and 1.2 V (cathodic-o-) accumulation potentials. Given these conditions, the linear working range was observed to be within 0.01-0.11 microg L(-1) (SW anodic and CV). The analytical detection limit was determined to be SW anodic and CV: 4.0 microg L(-1) (2.1 x 10(-11) mol L(-1)) dopamin, and the relative standard deviation at the dopamine concentration of SW anodic 0.05 microg L(-1) was 0.02% (n=15) at the optimum conditions.
一种制备简单的固定在碳纳米管糊电极（CNTPE）上的DNA被用于采用循环伏安法（CV）和方波（SW）溶出伏安法监测多巴胺离子浓度。探寻了最佳分析条件。与其他常见伏安法相比，获得的结果具有非常低的检测限。发现最佳参数如下：pH值3.5、SW振幅0.48 V、频率71 Hz、积累时间5 s、增量电位0.01 V以及积累电位-1.3 V（阳极-*-）和1.2 V（阴极-o-）。在这些条件下，观察到线性工作范围在0.01 - 0.11 μg L⁻¹（SW阳极和CV）之内。分析检测限确定为SW阳极和CV：4.0 μg L⁻¹（2.1×10⁻¹¹ mol L⁻¹）多巴胺，在最佳条件下，SW阳极多巴胺浓度为0.05 μg L⁻¹时的相对标准偏差为0.02%（n = 15）。
