使用碳纳米管微传感器对胃细胞中幽门螺杆菌DNA感染进行的病理体内分析。

Pathological In Vivo Analysis of Helicobacter DNA Infection in Stomach Cells Using Carbon Nanotube Microsensor.

作者信息

Lee Kyung, Jun Sihyun, Oh Yeseul, Lee Seojun, Oh Ye Jun, Kim Keum Sook, Ly Suw Young

机构信息

Biosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of Korea.

University of Michigan College of Pharmacy, 428 Church St, Ann Arbor, MI 48109, USA.

出版信息

Microorganisms. 2024 Dec 8;12(12):2531. doi: 10.3390/microorganisms12122531.

DOI:10.3390/microorganisms12122531

PMID:39770734

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11677723/

Abstract

The WHO has classified Helicobacter pylori as a group 1 carcinogen for stomach cancer since early 1994. However, despite the high prevalence of Helicobacter pylori infection, only about 3% of infected people eventually develop gastric cancer.Biomolecular detections of Helicobacter pylori(HP) were compared using specially modified sensors and fluorine immobilized on a carbon nanotube (HFCNT) electrode, which yielded sensitive results. Handheld voltammetric circuits were used for optimization. An anodic voltammogram of HP molecular oxidation was obtained at 0.0 V ± 0.1 (versus the Ag/AgCl/KCl) in a 0.1 ± 0.2 M NHHPO electrolyte solution. Under optimized conditions, the analytical working range was 2.98 × 10-22.127 × 10 CFU/mL HP using square wave (SW) stripping voltammetry, precision of R = 0.9857 ± 0.0005 (SWSV), the detection limit approached to 2.5 × 10 CFU/mL HP (S/N = 3).The developed techniques have been applied to diagnosis of early-stage HP infections using stomach tissue from healthy humans and gastric patients.

摘要

自1994年初以来，世界卫生组织已将幽门螺杆菌列为胃癌的1类致癌物。然而，尽管幽门螺杆菌感染率很高，但只有约3%的感染者最终会患上胃癌。使用专门改良的传感器和固定在碳纳米管（HFCNT）电极上的氟对幽门螺杆菌（HP）进行生物分子检测，结果很灵敏。使用手持式伏安电路进行优化。在0.1±0.2 M磷酸氢二铵电解质溶液中，在0.0 V±0.1（相对于Ag/AgCl/KCl）下获得了HP分子氧化的阳极伏安图。在优化条件下，使用方波（SW）溶出伏安法，分析工作范围为2.98×10⁻²至2.127×10 CFU/mL HP，精密度R = 0.9857±0.0005（SWSV），检测限接近2.5×10 CFU/mL HP（S/N = 3）。所开发的技术已应用于使用健康人和胃病患者的胃组织诊断早期HP感染。