Lee Kyung, Jun Sihyun, Oh Yeseul, Lee Seojun, Oh Ye Jun, Kim Keum Sook, Ly Suw Young
Biosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of Korea.
University of Michigan College of Pharmacy, 428 Church St, Ann Arbor, MI 48109, USA.
Microorganisms. 2024 Dec 8;12(12):2531. doi: 10.3390/microorganisms12122531.
The WHO has classified Helicobacter pylori as a group 1 carcinogen for stomach cancer since early 1994. However, despite the high prevalence of Helicobacter pylori infection, only about 3% of infected people eventually develop gastric cancer.Biomolecular detections of Helicobacter pylori(HP) were compared using specially modified sensors and fluorine immobilized on a carbon nanotube (HFCNT) electrode, which yielded sensitive results. Handheld voltammetric circuits were used for optimization. An anodic voltammogram of HP molecular oxidation was obtained at 0.0 V ± 0.1 (versus the Ag/AgCl/KCl) in a 0.1 ± 0.2 M NHHPO electrolyte solution. Under optimized conditions, the analytical working range was 2.98 × 10-22.127 × 10 CFU/mL HP using square wave (SW) stripping voltammetry, precision of R = 0.9857 ± 0.0005 (SWSV), the detection limit approached to 2.5 × 10 CFU/mL HP (S/N = 3).The developed techniques have been applied to diagnosis of early-stage HP infections using stomach tissue from healthy humans and gastric patients.
自1994年初以来，世界卫生组织已将幽门螺杆菌列为胃癌的1类致癌物。然而，尽管幽门螺杆菌感染率很高，但只有约3%的感染者最终会患上胃癌。使用专门改良的传感器和固定在碳纳米管（HFCNT）电极上的氟对幽门螺杆菌（HP）进行生物分子检测，结果很灵敏。使用手持式伏安电路进行优化。在0.1±0.2 M磷酸氢二铵电解质溶液中，在0.0 V±0.1（相对于Ag/AgCl/KCl）下获得了HP分子氧化的阳极伏安图。在优化条件下，使用方波（SW）溶出伏安法，分析工作范围为2.98×10⁻²至2.127×10 CFU/mL HP，精密度R = 0.9857±0.0005（SWSV），检测限接近2.5×10 CFU/mL HP（S/N = 3）。所开发的技术已应用于使用健康人和胃病患者的胃组织诊断早期HP感染。
