He Xin-Jian, Fassler Jan S
Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA.
Mol Microbiol. 2005 Dec;58(5):1454-67. doi: 10.1111/j.1365-2958.2005.04917.x.
The Saccharomyces cerevisiae Yap1p and Skn7p transcription factors collaborate in the activation of oxidative stress response (OSR) genes. Although Yap1p and Skn7p oxidative stress response elements (YRE, OSRE) have been characterized and identified in some OSR genes, many OSR genes lack such elements. In this study, the complex, oxidative responsive, CCP1 promoter was used as a model to investigate the cis-acting elements responsible for activation by oxidative stress. In addition to consensus YRE and OSRE sequences, novel Yap1p and Skn7p binding sites were identified in the CCP1 promoter. These new sites were found to mediate Yap1p- and Skn7p-dependent activation of OSR genes including TSA1 and CTT1 previously thought to lack Yap1p and Skn7p binding sites. The novel YREs and OSREs were found to be enriched in the promoter regions of a set of 179 OSR genes. The widespread existence of novel Yap1p and Skn7p binding sites strongly suggest that direct binding of Yap1p and Skn7p is responsible for activation of many more OSR genes than previously believed.
酿酒酵母Yap1p和Skn7p转录因子在氧化应激反应（OSR）基因的激活过程中协同作用。尽管Yap1p和Skn7p氧化应激反应元件（YRE、OSRE）已在一些OSR基因中得到表征和鉴定，但许多OSR基因缺乏此类元件。在本研究中，复杂的、氧化应激反应性的CCP1启动子被用作模型，以研究负责氧化应激激活的顺式作用元件。除了共有YRE和OSRE序列外，在CCP1启动子中还鉴定出了新的Yap1p和Skn7p结合位点。这些新位点被发现介导了包括TSA1和CTT1在内的OSR基因的Yap1p和Skn7p依赖性激活，而TSA1和CTT1此前被认为缺乏Yap1p和Skn7p结合位点。新的YRE和OSRE在一组179个OSR基因的启动子区域中富集。新的Yap1p和Skn7p结合位点的广泛存在强烈表明，Yap1p和Skn7p的直接结合对更多OSR基因的激活负责，其数量超过先前的认知。
