Nimmo Wilkie J S, Yager J A, Wilkie B N, Pascoe P J
Department of Pathology, Ontario Veterinary College, University of Guelph, Canada.
Vet Immunol Immunopathol. 1992 May;32(3-4):325-38. doi: 10.1016/0165-2427(92)90054-t.
Experimentally-induced type 1 hypersensitivities were induced in normal dogs to either ovalbumin or Ascaris antigen. In vitro and in vivo cell-mediated immune responses were measured before sensitization and again at 1 and 6 days after induction of anaphylaxis by intravenous challenge with antigen. Histamine-modulated lymphocyte functions, such as histamine-induced suppression, histamine co-mitogen induced blastogenesis and the in vivo cutaneous responses to intradermally injected mitogens decreased post anaphylaxis. Spontaneous suppression of the autologous mixed-lymphocyte reaction increased post anaphylaxis. Lymphocyte blastogenic response to Concanavalin A (Con A) decreased at 6 (but not at 1) days post anaphylaxis probably due to a mediator other than histamine. Blastogenesis of 24 h preincubated cells by suboptimal concentration of Con A, declined post anaphylaxis, but Con A-induced suppression was not significantly altered. Dogs with atopic dermatitis have some altered cell-mediated immune responses. Altered histamine-induced and spontaneous suppression, histamine suppression of mitogenesis and decreased contact sensitivity observed in this experimental type 1 hypersensitivity mimicked that of atopic dogs. Increased cutaneous response to mitogens observed in atopic dogs was not reproduced in the type 1 hypersensitive dogs. These findings suggest some of the altered cell-mediated immune functions observed in dogs with atopic dermatitis result from type 1 hypersensitivity. The other abnormalities may be intrinsic to the atopic state.
在正常犬中诱导实验性1型超敏反应，使其针对卵清蛋白或蛔虫抗原产生反应。在致敏前以及通过抗原静脉内激发诱导过敏反应后的第1天和第6天，分别测量体外和体内细胞介导的免疫反应。过敏反应后，组胺调节的淋巴细胞功能，如组胺诱导的抑制作用、组胺共刺激原诱导的细胞增殖以及对皮内注射促有丝分裂原的体内皮肤反应均降低。过敏反应后，自体混合淋巴细胞反应的自发抑制作用增强。过敏反应后第6天（而非第1天），淋巴细胞对刀豆球蛋白A（Con A）的增殖反应降低，这可能是由于组胺以外的介质所致。用次优浓度的Con A对预先孵育24小时的细胞进行刺激，过敏反应后细胞增殖下降，但Con A诱导的抑制作用无明显改变。患有特应性皮炎的犬有一些细胞介导的免疫反应改变。在这种实验性1型超敏反应中观察到的组胺诱导的和自发的抑制作用改变、组胺对有丝分裂的抑制作用以及接触敏感性降低，与特应性犬相似。在1型超敏反应犬中未再现特应性犬中观察到的对促有丝分裂原的皮肤反应增强。这些发现表明，在患有特应性皮炎的犬中观察到的一些细胞介导的免疫功能改变是由1型超敏反应引起的。其他异常可能是特应性状态所固有的。
