Paulík S, Mojzisová J, Bajová V, Baranová D, Paulíková I
University of Veterinary Medicine, Department of Infectology, Kosice, Slovak Republic.
Vet Med (Praha). 1996 Jan;41(1):7-12.
The mitogen induced blastogenic response of lymphocytes from normal dogs and dogs with generalized demodicosis (GD) was measured by the ethidium bromide (EB) fluorescence assay. Serum from GD dogs significantly suppressed the in vitro reactivity to Con A of peripheral blood lymphocytes (PBL) from normal dogs and GD dogs, however with a different percentage of suppression 40.6 and 81.2%, respectively. As a result, the degree of lymphocyte blastogenesis suppression in GD dogs did not parallel the immunosuppressive potency of their serum (Tab. IV). The data indicate that PBL obtained from GD dogs did not respond to Con A as well in the presence of serum from normal dogs as did PBL from normal dogs (Tab. IV). In one third of examined GD dogs a similar situation was described also by Hirsh et al. (1975). The basis for this cellular modified response is unknown. It does not appear that the age or the chronicity of the disease are related to this observation. Further studies are necessary to elucidate this relation. The GD dogs showed not only a significant depression of the lymphocyte response to Con A but also enhancement of the ability of unstimulated cells to proliferate was also observed (Tab. IV). Similar observation was reported by others (Barriga et al., 1992). The meaning of this is not clear at present. This finding is discussed in the light of proposed different effects of the parasite or the host's reactivity to the parasite on different subsets of lymphocytes. No significant difference of PBL responsiveness to Con A between healthy dogs with respect to the age (Tab. III) and the time of examination (compare results in Tabs. I and IV) was observed. Autologous serum showed a better responsiveness of normal canine lymphocytes to Con A than fetal calf serum (FCS). It is suggested that the use of FCS might lead to an erroneous judgement (Tab. I). Both lectins, Con A and PHA induced cell proliferation of healthy dogs in very similar amount (Tab. II). Our results indicated that EB fluorescence assay is a useful method for detection a respondence of canine lymphocyte blastogenesis.
采用溴化乙锭（EB）荧光分析法测定正常犬和患有全身性蠕形螨病（GD）犬淋巴细胞的丝裂原诱导增殖反应。GD犬的血清显著抑制正常犬和GD犬外周血淋巴细胞（PBL）对刀豆蛋白A（Con A）的体外反应性，但抑制百分比不同，分别为40.6%和81.2%。因此，GD犬淋巴细胞增殖抑制程度与其血清的免疫抑制效力不平行（表IV）。数据表明，在正常犬血清存在的情况下，从GD犬获得的PBL对Con A的反应不如正常犬的PBL（表IV）。在三分之一的受试GD犬中，Hirsh等人（1975年）也描述了类似情况。这种细胞反应改变的基础尚不清楚。似乎疾病的年龄或慢性程度与该观察结果无关。需要进一步研究以阐明这种关系。GD犬不仅对Con A的淋巴细胞反应显著降低，还观察到未刺激细胞增殖能力增强（表IV）。其他人也报道了类似观察结果（Barriga等人，1992年）。目前其意义尚不清楚。根据寄生虫或宿主对寄生虫的反应对淋巴细胞不同亚群的不同影响来讨论这一发现。未观察到健康犬的PBL对Con A的反应在年龄（表III）和检查时间方面（比较表I和IV中的结果）有显著差异。自体血清显示正常犬淋巴细胞对Con A的反应性优于胎牛血清（FCS）。建议使用FCS可能会导致错误判断（表I）。两种凝集素，Con A和PHA诱导健康犬细胞增殖的量非常相似（表II）。我们的结果表明，EB荧光分析法是检测犬淋巴细胞增殖反应的一种有用方法。
