Wang Z, Shao Y, Guo Y, Yuan J
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Transbound Emerg Dis. 2008 Dec;55(9-10):369-76. doi: 10.1111/j.1865-1682.2008.01049.x. Epub 2008 Aug 27.
The experiment was conducted to evaluate the effect of dietary supplementation with 0, 50, 100 and 200 mg/kg of beta-1,3/1,6-glucan extracted from Saccharomyces cerevisiae on cellular immune responses and classical swine fever virus (CSFV) antibody production of weaned piglets. Thirty-two (Landrace x Large White; 26 days old; 7.05+/-0.25 kg body weight) castrated male piglets were randomly allocated to four treatments, eight pigs per treatment. All piglets were vaccinated with live-attenuated Chinese strain cell vaccines at 26 days of age. The results showed that dietary supplementation with beta-1,3/1,6-glucan could significantly (P<0.01) enhance peripheral blood lymphocyte proliferation activity in weaned piglets after 14 days administration, whereas no significant effect (P>0.05) was observed on day 28. Dietary beta-1,3/1,6-glucan supplementation could significantly enhance CSFV antibody blocking rate (P<0.05) on days 14 and 21 after inoculation. Although dietary beta-1,3/1,6-glucan supplementation did not significantly influence (P>0.05) lymphocyte subgroups on day 14, high levels of beta-1,3/1,6-glucan (100 and 200 mg/kg) increased the percentage of cytotoxic/suppressor lymphocytes (CD8(+)) significantly (P<0.05), decreased the ratio of CD4(+)/CD8(+) significantly (P<0.05) and increased the percentage of total T lymphocytes (CD3(+)) on day 28. These results suggest that beta-1,3/1,6-glucan may have a beneficial effect on the immune system of swine, and dietary beta-1,3/1,6-glucan supplementation had a transient impact on lymphocyte proliferation activity. The CSFV antibody blocking rate and the percentage of peripheral blood CD8(+) T cells could be enhanced by continuous beta-1,3/1,6-glucan supplementation. These findings support the potential application of beta-1,3/1,6-glucan as a prophylactic agent in increasing animal immune functions and controlling CSF disease.
本试验旨在评估日粮中添加0、50、100和200mg/kg从酿酒酵母中提取的β-1,3/1,6-葡聚糖对断奶仔猪细胞免疫反应和经典猪瘟病毒（CSFV）抗体产生的影响。32头（长白猪×大白猪；26日龄；体重7.05±0.25kg）去势公仔猪被随机分为4组，每组8头。所有仔猪在26日龄时接种中国株减毒活细胞疫苗。结果表明，日粮中添加β-1,3/1,6-葡聚糖在给药14天后可显著（P<0.01）提高断奶仔猪外周血淋巴细胞增殖活性，而在第28天未观察到显著影响（P>0.05）。日粮添加β-1,3/1,6-葡聚糖可在接种后第14天和第21天显著提高CSFV抗体阻断率（P<0.05）。虽然日粮添加β-1,3/1,6-葡聚糖在第14天对淋巴细胞亚群没有显著影响（P>0.05），但高水平的β-1,3/1,6-葡聚糖（100和200mg/kg）在第28天显著增加了细胞毒性/抑制性淋巴细胞（CD8(+)）的百分比（P<0.05），显著降低了CD4(+)/CD8(+)的比值（P<0.05），并增加了总T淋巴细胞（CD3(+)）的百分比。这些结果表明，β-1,3/1,6-葡聚糖可能对猪的免疫系统有有益作用，日粮添加β-1,3/1,6-葡聚糖对淋巴细胞增殖活性有短暂影响。持续添加β-1,3/1,6-葡聚糖可提高CSFV抗体阻断率和外周血CD8(+) T细胞百分比。这些发现支持了β-1,3/1,6-葡聚糖作为预防性药物在提高动物免疫功能和控制猪瘟疾病方面的潜在应用。
