来自绵毛水苏的两种酰化黄酮苷及其自由基清除活性。

Two acylated flavonoid glycosides from Stachys bombycina, and their free radical scavenging activity.

作者信息

Delazar A, Celik S, Göktürk R S, Unal O, Nahar L, Sarker S D

机构信息

School of Pharmacy and Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

出版信息

Pharmazie. 2005 Nov;60(11):878-80.

PMID:16320954

Abstract

Preparative reversed-phase HPLC analysis of the methanol extract of the aerial parts of Stachys bombycina (Lamiaceae) afforded two acylated flavonoids glycosides, chrysoeriol 7-O-[6-O-acetyl-beta-D-allopyranosyl]-(1 --> 2)-beta-D-glucopyranoside (1) and apigenin 7-O-beta-D-(6-p-coumaroyl)-glucopyranoside (2), the former being a new natural product. The structures of these compounds were elucidated unambiguously by UV spectroscopic analyses using shift reagents, ESIMS, and 1D and 2D NMR spectroscopic techniques. The free radical scavenging activity of 1 and 2 compounds were assessed by DPPH assay, and the RC50 values were 1.25 x 10(-2) and 7.69 x 10(-4) mg/mL, respectively.

摘要

对绵毛水苏（唇形科）地上部分的甲醇提取物进行制备型反相高效液相色谱分析，得到了两种酰化黄酮苷，即木犀草素7 - O - [6 - O - 乙酰基 - β - D - 阿洛吡喃糖基] - (1→2) - β - D - 吡喃葡萄糖苷（1）和芹菜素7 - O - β - D - (6 - 对香豆酰基) - 吡喃葡萄糖苷（2），前者是一种新的天然产物。通过使用位移试剂的紫外光谱分析、电喷雾离子化质谱（ESIMS）以及一维和二维核磁共振光谱技术，明确阐明了这些化合物的结构。通过二苯基苦味酰基自由基（DPPH）测定法评估了化合物1和2的自由基清除活性，其半数清除浓度（RC50）值分别为1.25×10⁻²和7.69×10⁻⁴mg/mL。