Crawford R L
J Bacteriol. 1975 Feb;121(2):531-6. doi: 10.1128/jb.121.2.531-536.1975.
A species of Bacillus, tentatively identified as B. circulans, degrades protocatechuic acid by a novel reaction involving meta-fission between C2 and C3 of the benzene nucleus. 2-Hydroxymuconic semialdehyde is then degraded to pyruvate and acetaldehyde by enzymatic reactions described in previous work. Protocatechuate 2,3-oxygenase exhibits a rather narrow substrate specificity; the methyl and ethyl esters of protocatechuic acid are oxidized, but other substrates for ring-fission oxygenases, notably catechol, gallic acid, and homoprotocatechuic acid, are not attached.
一种暂定为环状芽孢杆菌的芽孢杆菌属细菌,通过一种涉及苯环C2和C3之间间位裂解的新反应降解原儿茶酸。然后,2-羟基粘康酸半醛通过先前工作中描述的酶促反应降解为丙酮酸和乙醛。原儿茶酸2,3-加氧酶表现出相当窄的底物特异性;原儿茶酸的甲酯和乙酯可被氧化,但环裂解加氧酶的其他底物,特别是儿茶酚、没食子酸和高原儿茶酸,则不被作用。