Kato Souichiro, Haruta Shin, Cui Zong Jun, Ishii Masaharu, Igarashi Yasuo
Department of Biotechnology, Laboratory of Applied Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Tokyo 113-8657, Japan.
FEMS Microbiol Ecol. 2004 Dec 27;51(1):133-42. doi: 10.1016/j.femsec.2004.07.015.
A stable cellulose-degrading microflora enriched from composting materials has been analyzed in our laboratory. Cellulose-degrading efficiency of an anaerobic cellulolytic isolate, Clostridium straminisolvens CSK1, was remarkably lower than that of the original microflora. We successfully constructed bacterial communities with effective cellulose degradation by mixing C. straminisolvens CSK1 with aerobic non-cellulolytic bacteria isolated from the original microflora. Comparison of the cellulose degradation processes of the pure culture of C. straminisolvens CSK1 and the mixed-culture indicated that non-cellulolytic bacteria essentially contribute to cellulose degradation by supplying anaerobic environment, consuming metabolites, which otherwise deteriorate the cellulolytic activity, and by neutralizing pH.
我们实验室对从堆肥材料中富集得到的稳定纤维素降解微生物群落进行了分析。厌氧纤维素分解菌菌株 Clostridium straminisolvens CSK1 的纤维素降解效率显著低于原始微生物群落。通过将 Clostridium straminisolvens CSK1 与从原始微生物群落中分离得到的好氧非纤维素分解菌混合，我们成功构建了具有高效纤维素降解能力的细菌群落。对 Clostridium straminisolvens CSK1 纯培养物和混合培养物的纤维素降解过程进行比较表明，非纤维素分解菌通过提供厌氧环境、消耗否则会降低纤维素分解活性的代谢产物以及中和 pH 值，对纤维素降解起到了重要作用。
