College of Chemistry and Life Science/Research Institute for New Energy, China Three Gorges University, Yichang, 443002, China.
Appl Biochem Biotechnol. 2013 Dec;171(7):1578-88. doi: 10.1007/s12010-013-0431-8. Epub 2013 Aug 24.
The effect of a non-cellulolytic bacterium W2-10 (Geobacillus sp.) on the cellulose-degrading activity of a cellulolytic bacterium CTL-6 (Clostridium thermocellum) was determined using cellulose materials (paper and straw) in peptone cellulose solution (PCS) medium under aerobic conditions. The results indicated that in the co-culture, addition of W2-10 resulted in a balanced medium pH, and may provide the required anaerobic environment for CTL-6. Overall, addition of W2-10 was beneficial to CTL-6 growth in the adverse environment of the PCS medium. In co-culture with W2-10, the CTL-6 cellulose degradation efficiency of filter paper and alkaline-treated wheat straw significantly increased up to 72.45 and 37.79 %, respectively. The CMCase activity and biomass of CTL-6 also increased from 0.23 U ml(-1) and 45.1 μg ml(-1) (DNA content) up to 0.47 U ml(-1) and 112.2 μg ml(-1), respectively. In addition, co-culture resulted in accumulation of acetate and propionate up to 4.26 and 2.76 mg ml(-1). This was a respective increase of 2.58 and 4.45 times, in comparison to the monoculture with CTL-6.
采用纤维素材料（滤纸和稻草）在蛋白胨纤维素溶液（PCS）培养基中，在有氧条件下，研究了一种非纤维素分解菌 W2-10（芽孢杆菌属）对纤维素分解菌 CTL-6（产热梭菌）纤维素降解活性的影响。结果表明，在共培养中，添加 W2-10 可使培养基 pH 保持平衡，并可能为 CTL-6 提供所需的厌氧环境。总的来说，添加 W2-10 有利于 CTL-6 在 PCS 培养基不利环境中的生长。与 W2-10 共培养时，滤纸和碱性处理麦草的 CTL-6 纤维素降解效率分别显著提高到 72.45%和 37.79%。CTL-6 的 CMCase 活性和生物量也分别从 0.23 U ml(-1)和 45.1 μg ml(-1)（DNA 含量）增加到 0.47 U ml(-1)和 112.2 μg ml(-1)。此外，共培养导致乙酸和丙酸的积累分别达到 4.26 和 2.76 mg ml(-1)。与 CTL-6 的单一培养相比，分别增加了 2.58 倍和 4.45 倍。
