Thiry Julien, Tempesta Maria, Camero Michele, Tarsitano Elvira, Bellacicco Anna Lucia, Thiry Etienne, Buonavoglia Canio
Department of Animal Health and Well-being, Faculty of Veterinary Medicine, University of Bari, 70010 Valenzano, Italy.
Vet Microbiol. 2006 Mar 31;113(3-4):303-8. doi: 10.1016/j.vetmic.2005.11.001. Epub 2005 Dec 2.
Taking into account the close antigenic relationship between bovine herpesvirus 1 (BoHV-1) and caprine herpesvirus 1 (CpHV-1), a live attenuated glycoprotein E (gE) negative BoHV-1 vaccine was assessed in goats with the aim to protect against CpHV-1 infection. Vaccine safety was evaluated by intranasal inoculation of two groups of goats with either a gE-negative BoHV-1 vaccine or a virulent BoHV-1. The length of viral excretion and the peak viral titre were reduced with the gE-negative vaccine. To assess the efficacy, two goats were inoculated intranasally twice 2 weeks apart with a gE-negative BoHV-1 vaccine. Four weeks later, immunised and control goats were challenged with CpHV-1. A 2 log(10) reduction in the peak viral titre was observed and the challenge virus excretion lasted 2 days more in immunised than in control goats. These data indicate the safety and the partial efficacy of a live attenuated gE-negative BoHV-1 vaccine intranasally administrated in goats.
考虑到牛疱疹病毒1型（BoHV-1）和山羊疱疹病毒1型（CpHV-1）之间密切的抗原关系，对一种减毒活的糖蛋白E（gE）阴性BoHV-1疫苗在山羊中进行了评估，旨在预防CpHV-1感染。通过给两组山羊分别经鼻接种gE阴性BoHV-1疫苗或强毒BoHV-1来评估疫苗安全性。使用gE阴性疫苗时，病毒排泄时间和病毒滴度峰值均降低。为评估疗效，给两只山羊经鼻接种gE阴性BoHV-1疫苗，间隔2周接种两次。四周后，对免疫山羊和对照山羊用CpHV-1进行攻毒。观察到免疫山羊的病毒滴度峰值降低了2个对数（10），且攻毒病毒排泄时间比对照山羊长2天。这些数据表明经鼻接种的减毒活gE阴性BoHV-1疫苗在山羊中的安全性和部分疗效。
