Leibold W, Flanagan T D, Menezes J, Klein G
J Natl Cancer Inst. 1975 Jan;54(1):65-8. doi: 10.1093/jnci/54.1.65.
Human lymphoid cells isolated from the peripheral blood of adults, from cord blood, and from fetal liver, spleen, bone marrow, and thymus were cultivated with or without a cell-free preparation of Epstein-Barr virus (EBV) with demonstrated transforming activity. The cultures were examined for the EBV-associated nuclear antigen (EBNA) and for transfromation into permanent lymphoblastoid cell lines (LCL). EBNA, seen only in cultures that had received exogenous EBV, was detected between days 1 and 6 after addition of EBV, most frequently on day 3. EBNA-positive cells had a lymphoblastoid appearance. Transformation into established LCL became apparent between days 12 and 19. The addition of pokeweed mitogen to cultures containing EBV enhanced the development of EBNA, whereas phytohemagglutinin or concanavalin A had no such effect. Neither EBNA nor transfomration was observed in lymphoid cells from fetal thymus. In fetal spleen, bone marrow, and liver cells, EBV regularly induced EBNA and LCL transformation.
从成人外周血、脐带血以及胎儿肝脏、脾脏、骨髓和胸腺中分离出的人类淋巴细胞，在有或没有已证明具有转化活性的爱泼斯坦 - 巴尔病毒（EBV）无细胞制剂的情况下进行培养。对培养物进行检查，以检测EBV相关核抗原（EBNA）以及是否转化为永久性淋巴母细胞系（LCL）。EBNA仅在接受外源性EBV的培养物中可见，在添加EBV后的第1至6天被检测到，最常见于第3天。EBNA阳性细胞具有淋巴母细胞样外观。在第12至19天之间，转化为已建立的LCL变得明显。向含有EBV的培养物中添加商陆有丝分裂原可增强EBNA的产生，而植物血凝素或刀豆球蛋白A则没有这种作用。在胎儿胸腺的淋巴细胞中未观察到EBNA和转化现象。在胎儿脾脏、骨髓和肝细胞中，EBV regularly诱导EBNA和LCL转化。 （注：“regularly”原英文有误，这里按正确语境翻译为“经常” ）
