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In-house nucleic acid amplification assays in research: how much quality control is needed before one can rely upon the results?

作者信息

Apfalter Petra, Reischl Udo, Hammerschlag Margaret R

机构信息

Department of Clinical Microbiology, Institute of Hygiene and Medical Microbiology, Vienna General Hospital, Waehringer Guertel 18-20/5P, 1090 Vienna, Austria.

出版信息

J Clin Microbiol. 2005 Dec;43(12):5835-41. doi: 10.1128/JCM.43.12.5835-5841.2005.

DOI:10.1128/JCM.43.12.5835-5841.2005
PMID:16333064
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1317225/
Abstract
摘要

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本文引用的文献

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The presence of Propionibacterium spp. in the vitreous fluid of uveitis patients with sarcoidosis.结节病性葡萄膜炎患者玻璃体液中丙酸杆菌属的存在情况。
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Herpes simplex virus as a determinant risk factor for coronary artery atherosclerosis and myocardial infarction.单纯疱疹病毒作为冠状动脉粥样硬化和心肌梗死的决定性危险因素。
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Confidence in polymerase chain reaction diagnosis can be improved by Bayesian estimation of post-test disease probability.通过贝叶斯估计检测后疾病概率，可以提高对聚合酶链反应诊断的信心。
J Clin Epidemiol. 2005 Mar;58(3):252-60. doi: 10.1016/j.jclinepi.2004.06.012.
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External quality assessment for molecular detection of Bordetella pertussis in European laboratories.欧洲实验室中百日咳博德特氏菌分子检测的外部质量评估。
J Clin Microbiol. 2005 Jan;43(1):30-5. doi: 10.1128/JCM.43.1.30-35.2005.
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Involvement of Chlamydia pneumoniae in atherosclerosis: more evidence for lack of evidence.肺炎衣原体与动脉粥样硬化的关联：更多缺乏证据的证据。
J Clin Microbiol. 2005 Jan;43(1):19-24. doi: 10.1128/JCM.43.1.19-24.2005.
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Detection of Chlamydophila pneumoniae in the bone marrow of two patients with unexplained chronic anaemia.两名不明原因慢性贫血患者骨髓中肺炎衣原体的检测。
Eur J Haematol. 2005 Jan;74(1):77-83. doi: 10.1111/j.1600-0609.2004.00353.x.
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Demonstration of intracellular microorganisms (Rickettsia spp., Chlamydia pneumoniae, Bartonella spp.) in pathological human aortic valves by PCR.通过聚合酶链反应（PCR）在病理性人类主动脉瓣中检测细胞内微生物（立克次氏体属、肺炎衣原体、巴尔通体属）
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