Yasuhara Toru, Tada Rei, Nakano Yukiko, Tei Mamoru, Mochida Chikako, Kamei Motohiro, Kinoshita Shigeru
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Acta Ophthalmol Scand. 2005 Jun;83(3):364-9. doi: 10.1111/j.1600-0420.2005.00449.x.
An immunological reaction to a bacterial antigen, such as Mycobacterium tuberculosis or Propionibacterium spp., is suspected to be an initial mechanism in the disorder known as sarcoidosis. We investigated whether or not P. acnes, P. granulosum or M. tuberculosis are present in the vitreous fluid of eyes suffering from uveitis with sarcoidosis.
Using polymerase chain reaction, we analysed the presence of P. acnes, P. granulosum and/or M. tuberculosis DNA in vitreous samples taken from six eyes with sarcoidosis and six control eyes.
Among the six uveitis eyes with sarcoidosis, we detected P. acnes DNA in two eyes, P. granulosum DNA in four eyes, and both P. acnes and P. granulosum DNA in one eye, but no Propionibacterium spp. in the control eyes. M. tuberculosis DNA was not present in any of the patient or control eyes.
This is the first report indicating the presence of Propionibacterium spp. and/or its DNA in the vitreous fluid of sarcoidic eyes with uveitis. This, therefore, supports the idea that Propionibacterium spp. are involved in the aetiology of uveitis in sarcoidosis.
对诸如结核分枝杆菌或丙酸杆菌属等细菌抗原的免疫反应被怀疑是结节病这种疾病的初始机制。我们研究了痤疮丙酸杆菌、颗粒丙酸杆菌或结核分枝杆菌是否存在于患有结节病性葡萄膜炎的眼睛的玻璃体液中。
我们使用聚合酶链反应分析了从6只患有结节病的眼睛和6只对照眼睛采集的玻璃体液样本中痤疮丙酸杆菌、颗粒丙酸杆菌和/或结核分枝杆菌DNA的存在情况。
在6只患有结节病的葡萄膜炎眼睛中，我们在2只眼睛中检测到痤疮丙酸杆菌DNA，在4只眼睛中检测到颗粒丙酸杆菌DNA，在1只眼睛中同时检测到痤疮丙酸杆菌和颗粒丙酸杆菌DNA，但在对照眼睛中未检测到任何丙酸杆菌属。在任何患者或对照眼睛中均未检测到结核分枝杆菌DNA。
这是第一份表明在患有结节病性葡萄膜炎的眼睛的玻璃体液中存在丙酸杆菌属和/或其DNA的报告。因此，这支持了丙酸杆菌属参与结节病性葡萄膜炎病因学的观点。
