Honda S, Fujioka T, Tokieda M, Gotoh T, Nishizono A, Nasu M
Second Dept. of Internal Medicine, Oita Medical University, Japan.
Scand J Gastroenterol. 1998 May;33(5):454-60. doi: 10.1080/00365529850171990.
Helicobacter pylori infection is associated with gastroduodenal disease in humans. In this study we aimed to show this relationship directly in Mongolian gerbils.
The animals were challenged orally with H. pylori and killed 1, 2, 3, and 6 months after inoculation for histologic and anti-H. pylori antibody titer examination.
The spiral bacteria were observed in the mucus and gastric pits of all infected animals. A severe infiltration of the lamina propria by polymorphonuclear and mononuclear cells was seen 1 month after H. pylori inoculation. The submucosa was infiltrated by mainly mononuclear cells with formation of lymphoid follicles. Erosion of the gastric mucosa appeared soon after inoculation, whereas gastric ulcers, gastritis cystica profunda, and atrophy with goblet cell metaplasia occurred between 3 and 6 months after inoculation. In the duodenal mucosa a mild inflammatory cell infiltration with ballooning and diminished number of duodenal glands was seen. The IgG anti-H. pylori antibody titer increased gradually after 2 months of inoculation.
Since the gastritis, gastric ulcers, atrophic gastritis, and intestinal metaplasia that developed in Mongolian gerbils were similar to those observed in humans, this model may be useful to study the therapy of gastric ulcer and, with a longer observation period, to confirm a possible relationship between H. pylori and malignancy.
幽门螺杆菌感染与人类胃十二指肠疾病相关。在本研究中，我们旨在在蒙古沙鼠中直接证实这种关系。
给动物口服接种幽门螺杆菌，并在接种后1、2、3和6个月处死，进行组织学检查和抗幽门螺杆菌抗体滴度检测。
在所有感染动物的黏液和胃小凹中均观察到螺旋菌。接种幽门螺杆菌1个月后，可见固有层有大量多形核细胞和单核细胞浸润。黏膜下层主要为单核细胞浸润，并形成淋巴滤泡。接种后不久出现胃黏膜糜烂，而胃溃疡、深部囊性胃炎和杯状细胞化生萎缩在接种后3至6个月出现。十二指肠黏膜可见轻度炎症细胞浸润，十二指肠腺出现气球样变且数量减少。接种2个月后，抗幽门螺杆菌IgG抗体滴度逐渐升高。
由于蒙古沙鼠发生的胃炎、胃溃疡、萎缩性胃炎和肠化生与人类观察到的相似，该模型可能有助于研究胃溃疡的治疗，并且在更长的观察期内，有助于证实幽门螺杆菌与恶性肿瘤之间可能存在的关系。
