2型单纯疱疹病毒对人类细胞DNA合成的诱导作用。
Induction of human cell DNA synthesis by herpes simplex virus type 2.
作者信息
Melvin P, Kucera L S
出版信息
J Virol. 1975 Mar;15(3):534-9. doi: 10.1128/JVI.15.3.534-539.1975.
The effect of herpes simplex virus type 2 (HSV-2) infection on the synthesis of DNA in human embryonic fibroblast cells was determined at temperatures permissive (37 C) and nonpermissive (42 C) for virus multiplication. During incubation of HSV-2 infected cultures at 42 C for 2 to 4 days or after shift-down from 42 to 37 C, incorporation of (3H)TdR into total DNA was increased 2-to 30-fold as compared with mock-infected cultures. Analysis of the (3H)DNA suggested that host cell DNA synthesis was induced by HSV-2 infection. Induction of host cell DNA synthesis by HSV-2 also occurred in cells arrested in DNA replication by low serum concentration. The three strains of HSV-2 tested were capable of stimulating cellular DNA synthesis. Virus inactivated by UV irradiation, heat, or neutral red dye and light did not induce cellular DNA synthesis, suggesting that an active viral genome is necessary for induction.
在允许病毒增殖的温度（37℃）和不允许病毒增殖的温度（42℃）下，测定了2型单纯疱疹病毒（HSV-2）感染对人胚成纤维细胞中DNA合成的影响。在42℃下将感染HSV-2的培养物孵育2至4天，或从42℃降至37℃后，与模拟感染的培养物相比，（3H）TdR掺入总DNA的量增加了2至30倍。对（3H）DNA的分析表明，宿主细胞DNA合成是由HSV-2感染诱导的。HSV-2对宿主细胞DNA合成的诱导也发生在因血清浓度低而停滞于DNA复制的细胞中。所测试的三株HSV-2都能够刺激细胞DNA合成。经紫外线照射、加热或中性红染料及光照灭活的病毒不会诱导细胞DNA合成，这表明活跃的病毒基因组是诱导所必需的。
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