Santhosh S, Sini T K, Anandan R, Mathew P T
Fish Processing Division, Central Institute of Fisheries Technology, Cochin 682 029, India.
Toxicology. 2006 Feb 15;219(1-3):53-9. doi: 10.1016/j.tox.2005.11.001. Epub 2005 Dec 5.
We have studied the protective effect of chitosan on isoniazid- and rifampicin-induced hepatotoxicity with respect to the changes in the levels of diagnostic marker enzymes (in serum), lipid components and lipid peroxidation (in serum and liver). The oral administration of antitubercular drugs caused a significant elevation in the levels of diagnostic marker enzymes and cholesterol, triglycerides, free fatty acids and lipid peroxidation in serum and liver of experimental rats. There was a slight decline in the level of phospholipids in liver tissue also observed. Co-administration of chitosan significantly prevented the antitubercular drugs-induced elevation in the levels of serum diagnostic marker enzymes (alanine amino transferase, aspartate amino transferase, lactate dehydrogenase, acid phosphatase and alkaline phosphatase) in experimental groups of rats. It exerted a significant antilipidemic effect against isoniazid- and rifampicin-induced hepatitis by maintaining the levels cholesterol, triglycerides, free fatty acids and phospholipids in serum and liver at near normalcy. A tendency to prevent the isoniazid- and rifampicin-induced lipid peroxidation was also observed. The results of the present study indicated that the hepatoprotective effect of chitosan might be ascribable to its antilipidemic effect and/or antioxidant property.
我们研究了壳聚糖对异烟肼和利福平诱导的肝毒性的保护作用，涉及诊断标志物酶（血清中）、脂质成分以及脂质过氧化（血清和肝脏中）水平的变化。口服抗结核药物导致实验大鼠血清和肝脏中的诊断标志物酶、胆固醇、甘油三酯、游离脂肪酸水平以及脂质过氧化显著升高。肝脏组织中的磷脂水平也略有下降。壳聚糖与抗结核药物共同给药显著预防了实验组大鼠血清诊断标志物酶（丙氨酸氨基转移酶、天冬氨酸氨基转移酶、乳酸脱氢酶、酸性磷酸酶和碱性磷酸酶）水平因抗结核药物诱导而升高。通过将血清和肝脏中的胆固醇、甘油三酯、游离脂肪酸和磷脂水平维持在接近正常水平，壳聚糖对异烟肼和利福平诱导的肝炎发挥了显著的抗血脂作用。还观察到壳聚糖有预防异烟肼和利福平诱导的脂质过氧化的趋势。本研究结果表明，壳聚糖的肝脏保护作用可能归因于其抗血脂作用和/或抗氧化特性。
