Gholamiandekhordi Ahmad R, Ducatelle Richard, Heyndrickx Marc, Haesebrouck Freddy, Van Immerseel Filip
Department of Pathology, Bacteriology and Avian Diseases, Research Group Veterinary Public Health and Zoonoses, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Vet Microbiol. 2006 Mar 10;113(1-2):143-52. doi: 10.1016/j.vetmic.2005.10.023. Epub 2005 Dec 5.
Due to the diminished use of growth-promoting antibiotics in the European Union, Clostridium perfringens induced necrotic enteritis and subclinical disease have become important threats to poultry health. A study was set up to genotypically and phenotypically characterise C. perfringens isolates from poultry flocks with different health status. Animals from healthy flocks were sampled by cloacal swabs, while intestinal and liver samples of animals suffering from necrotic enteritis were analysed. A total of 27 isolates was obtained from 23 broiler flocks without clinical problems and 36 isolates were obtained from 8 flocks with clinical problems. Using PFGE typing, high genetic diversity was detected between isolates from different flocks. Isolates derived from flocks where disease outbreaks occurred were clonal within each flock, but each flock harboured a different clone. All isolates were of toxin type A. Isolates from 5 out of 35 PFGE types carried the cpb2 gene, encoding the beta2 toxin, and isolates from 2 out of 35 PFGE types harboured the cpe gene, encoding the enterotoxin. In vitro alpha toxin production for all isolates was quantified by enzyme-linked immunosorbent assay. It was shown that in vitro alpha toxin production of C. perfringens isolates from diseased flocks was not higher than in vitro alpha toxin production from isolates derived from healthy flocks.
由于欧盟减少了促生长抗生素的使用，产气荚膜梭菌引起的坏死性肠炎和亚临床疾病已成为家禽健康的重要威胁。开展了一项研究，旨在从基因型和表型上对来自不同健康状况家禽群的产气荚膜梭菌分离株进行特征分析。对健康家禽群的动物进行泄殖腔拭子采样，同时对患有坏死性肠炎的动物的肠道和肝脏样本进行分析。从23个无临床问题的肉鸡群中总共获得了27株分离株，从8个有临床问题的鸡群中获得了36株分离株。使用脉冲场凝胶电泳（PFGE）分型，检测到不同鸡群的分离株之间存在高度遗传多样性。来自发生疾病暴发鸡群的分离株在每个鸡群内是克隆性的，但每个鸡群含有不同的克隆。所有分离株均为A型毒素型。35种PFGE类型中的5种类型的分离株携带编码β2毒素的cpb2基因，35种PFGE类型中的2种类型的分离株含有编码肠毒素的cpe基因。通过酶联免疫吸附测定法定量所有分离株的体外α毒素产量。结果表明，来自患病鸡群的产气荚膜梭菌分离株的体外α毒素产量不高于来自健康鸡群分离株的体外α毒素产量。
