从亚马逊双色叶泡蛙皮肤提取物中鉴定和表征两种皮啡肽。

Identification and characterization of two dermorphins from skin extracts of the Amazonian frog Phyllomedusa bicolor.

作者信息

Mignogna G, Severini C, Simmaco M, Negri L, Erspamer G F, Kreil G, Barra D

机构信息

Dipartimento di Scienze Biochimiche A. Rossi Fanelli, Università La Sapienza, Roma, Italy.

出版信息

FEBS Lett. 1992 May 11;302(2):151-4. doi: 10.1016/0014-5793(92)80427-i.

DOI:10.1016/0014-5793(92)80427-i

PMID:1633846

Abstract

Skin extracts of South American hylid frogs of the subfamily Phyllomedusinae contain dermorphins and deltorphins, opioid heptapeptides highly selective for either mu or delta receptors. In all these peptides, a D-amino acid is present in the second position. The structure of the precursors for Ala-deltorphins was recently deduced from cloned cDNAs derived from skin of Phyllomedusa bicolor (Richter et al. (1990) Proc. Natl. Acad. Sci. USA 87, 4836-4839). From the amino acid sequence of these precursors, the existence of three peptides related to dermorphin could be predicted. From methanol extracts of skin of Ph. bicolor we have isolated two of these peptides, [Lys7]dermorphin-OH and [Trp4,Asn7]dermorphin-OH. The biological activity of these new dermorphins and their amidated counterparts is presented.

摘要

叶泡蛙亚科的南美叶泡蛙皮肤提取物含有皮啡肽和δ-阿片肽，这两种阿片样七肽对μ受体或δ受体具有高度选择性。在所有这些肽中，第二个位置都存在一个D-氨基酸。最近，从双色叶泡蛙皮肤克隆的cDNA推导出了丙氨酰-δ-阿片肽前体的结构（Richter等人，（1990年）美国国家科学院院刊87，4836 - 4839）。根据这些前体的氨基酸序列，可以预测存在三种与皮啡肽相关的肽。从双色叶泡蛙皮肤的甲醇提取物中，我们分离出了其中两种肽，即[Lys7]皮啡肽 - OH和[Trp4,Asn7]皮啡肽 - OH。本文展示了这些新皮啡肽及其酰胺化对应物的生物活性。