Blednov Yuri A, Metten Pamela, Finn Deborah A, Rhodes Justin S, Bergeson Susan E, Harris R Adron, Crabbe John C
Waggoner Center for Alcohol and Addictions Research, University of Texas, Austin, TX 78712-0159, USA.
Alcohol Clin Exp Res. 2005 Nov;29(11):1949-58. doi: 10.1097/01.alc.0000187605.91468.17.
From several recent strain surveys (28 strains: Bachmanov et al., personal communication; 22 strains: Finn et al., unpublished), and from data in >100 other published studies of 24-hr two-bottle ethanol preference, it is known that male C57BL/6 (B6) mice self-administer about 10-14 g/kg/day and that female B6 mice self-administer about 12-18 g/kg/day. No strain has been found to consume more ethanol than B6. In one of our laboratories (Texas), we noted a markedly greater intake of ethanol in an F1 hybrid of B6 and FVB/NJ (FVB) mice.
To confirm and extend this finding, we repeated the study at another site (Portland) using concentrations up to 30% ethanol and also tested B6xFVB F1 mice in restricted access drinking procedures that produce high levels of alcohol intake.
At both sites, we found that B6xFVB F1 mice self-administered high levels of ethanol during two-bottle preference tests (females averaging from 20 to 35 g/kg/day, males 7-25 g/kg/day, depending on concentration). F1 hybrids of both sexes drank significantly more 20% ethanol than both the B6 and FVB strains. Female F1 hybrids also drank more 30% ethanol. In the restricted access tests, ethanol consumption in the F1 hybrids was equivalent to that in B6 mice.
These data show that this new genetic model has some significant advantages when compared to existing inbred strains, and could be used to explore the genetic basis of high ethanol drinking in mice.
从最近的几项品系调查（28个品系：巴赫马诺夫等人，个人交流；22个品系：芬恩等人，未发表）以及100多项其他已发表的24小时双瓶乙醇偏好研究的数据可知，雄性C57BL/6（B6）小鼠每天自行摄入约110 - 14克/千克乙醇，雌性B6小鼠每天自行摄入约12 - 18克/千克乙醇。尚未发现有品系比B6消耗更多的乙醇。在我们其中一个实验室（得克萨斯州），我们注意到B6和FVB/NJ（FVB）小鼠的F1杂交种对乙醇的摄入量明显更高。
为了证实并扩展这一发现，我们在另一个地点（波特兰）重复了该研究，使用了浓度高达30%的乙醇，并且还在限制饮水程序中对B6xFVB F1小鼠进行了测试，该程序会产生高水平的酒精摄入量。
在两个地点，我们都发现B6xFVB F1小鼠在双瓶偏好测试中自行摄入了高水平的乙醇（雌性平均每天20至35克/千克，雄性7至25克/千克，具体取决于浓度）。两种性别的F1杂交种饮用20%乙醇的量均显著多于B6和FVB品系。雌性F1杂交种饮用30%乙醇的量也更多。在限制饮水测试中，F1杂交种的乙醇消耗量与B6小鼠相当。
这些数据表明，与现有的近交系相比，这种新的遗传模型具有一些显著优势，可用于探索小鼠高乙醇摄入量的遗传基础。
