Panthee D R, Pantalone V R, Sams C E, Saxton A M, West D R, Orf J H, Killam A S
Department of Plant Sciences, University of Tennessee, 2431 Joe Johnson Dr, 252 Ellington Plant Sciences Bldg, Knoxville, TN 37996, USA.
Theor Appl Genet. 2006 Feb;112(3):546-53. doi: 10.1007/s00122-005-0161-6. Epub 2005 Dec 10.
Soybean [Glycine max (L.) Merr.] is the single largest source of protein in animal feed. However, a major limitation of soy proteins is their deficiency in sulfur-containing amino acids, methionine (Met) and cysteine (Cys). The objective of this study was to identify quantitative trait loci (QTL) associated with Met and Cys concentration in soybean seed. To achieve this objective, 101 F(6)-derived recombinant inbred lines (RIL) from a population developed from a cross of N87-984-16 x TN93-99 were used. Ground soybean seed samples were analyzed for Met and Cys concentration using a near infrared spectroscopy instrument. Data were analyzed using SAS software and QTL Cartographer. RIL differed (P<0.01) in Met and Cys concentrations, with a range of 5.1-7.3 (g kg(-1) seed dry weight) for Cys and 4.4-8.8 (g kg(-1) seed dry weight) for Met. Heritability estimates on an entry mean basis were 0.14 and 0.57 for Cys and Met, respectively. A total of 94 polymorphic simple sequence repeat molecular genetic markers were screened in the RIL. Single factor ANOVA was used to identify candidate QTL, which were confirmed by composite interval mapping using QTL Cartographer. Four QTL linked to molecular markers Satt235, Satt252, Satt427 and Satt436 distributed on three molecular linkage groups (MLG) D1a, F and G were associated with Cys and three QTL linked to molecular markers Satt252, Satt564 and Satt590 distributed on MLG F, G and M were associated with Met concentration in soybean seed. QTL associated with Met and Cys in soybean seed will provide important information to breeders targeting improvements in the nutritional quality of soybean.
大豆[Glycine max (L.) Merr.]是动物饲料中最大的单一蛋白质来源。然而，大豆蛋白的一个主要局限性是其含硫氨基酸甲硫氨酸（Met）和半胱氨酸（Cys）含量不足。本研究的目的是鉴定与大豆种子中Met和Cys浓度相关的数量性状位点（QTL）。为实现这一目标，使用了由N87 - 984 - 16与TN93 - 99杂交产生的群体中的101个F(6)衍生重组自交系（RIL）。使用近红外光谱仪分析磨碎的大豆种子样品中的Met和Cys浓度。数据使用SAS软件和QTL Cartographer进行分析。RIL的Met和Cys浓度存在差异（P<0.01），Cys的范围为5.1 - 7.3（g kg(-1)种子干重），Met的范围为4.4 - 8.8（g kg(-1)种子干重）。基于条目均值的遗传力估计，Cys和Met分别为0.14和0.57。在RIL中总共筛选了94个多态性简单序列重复分子遗传标记。使用单因素方差分析来鉴定候选QTL，通过使用QTL Cartographer的复合区间作图进行确认。分布在三个分子连锁群（MLG）D1a、F和G上的与分子标记Satt235、Satt252、Satt427和Satt436连锁的四个QTL与Cys相关，分布在MLG F、G和M上的与分子标记Satt252、Satt564和Satt590连锁的三个QTL与大豆种子中的Met浓度相关。与大豆种子中Met和Cys相关的QTL将为旨在改善大豆营养品质的育种者提供重要信息。
