Soybean Research Institute (Chinese Education Ministry's Key Laboratory of Soybean Biology), Northeast Agricultural University, 150030 Harbin, China.
Plant Cell Rep. 2010 Feb;29(2):125-31. doi: 10.1007/s00299-009-0804-1. Epub 2009 Dec 4.
High embryogenesis capacity of soybean (Glycine max (L.) Merr.) in vitro possessed potential for effective genetic engineering and tissue culture. The objects of this study were to identify quantitative trait loci (QTL) underlying embryogenesis traits and to identify genotypes with higher somatic embryogenesis capacity. A mapping population, consisting of 126 F(5:6) recombinant inbred lines, was advanced by single-seed-descent from cross between Peking (higher primary and secondary embryogenesis) and Keburi (lower primary and secondary embryogenesis). This population was evaluated for primary embryogenesis capacity from immature embryo cultures by measuring the frequency of somatic embryogenesis (FSE), the somatic embryo number per explant (EPE) and the efficiency of somatic embryogenesis (ESE). A total of 89 simple sequence repeat markers were used to construct a genetic linkage map. Six QTL were associated with somatic embryogenesis. Two QTL for FSE were found, QFSE-1 (Satt307) and QFSE-2 (Satt286), and both were located on linkage group C2 that explained 45.21 and 25.97% of the phenotypic variation, respectively. Four QTL for EPE (QEPE-1 on MLG H, QEPE-2 on MLG G and QEPE-3 on MLG G) were found, which explained 7.11, 7.56 and 6.12% of phenotypic variation, respectively. One QTL for ESE, QESE-1 (Satt427), was found on linkage group G that explained 6.99% of the phenotypic variation. QEPE-2 and QESE-1 were located in the similar region of MLG G. These QTL provide potential for marker assistant selection of genotypes with higher embryogenesis.
大豆（Glycine max (L.) Merr.）具有较高的胚胎发生能力，这使其在遗传工程和组织培养方面具有潜力。本研究的目的是鉴定与胚胎发生特性相关的数量性状基因座（QTL），并鉴定具有更高体细胞胚胎发生能力的基因型。通过单粒传代法，从 Peking（较高的初级和次级胚胎发生）和 Keburi（较低的初级和次级胚胎发生）之间的杂交中，衍生出一个由 126 个 F(5:6)重组自交系组成的作图群体。该群体通过测量体胚发生频率（FSE）、每个外植体的体胚数（EPE）和体胚发生效率（ESE），对来自未成熟胚培养的初级胚胎发生能力进行评估。总共使用了 89 个简单序列重复标记来构建遗传连锁图谱。共检测到与体细胞胚胎发生相关的 6 个 QTL。发现了两个与 FSE 相关的 QTL，QFSE-1（Satt307）和 QFSE-2（Satt286），均位于连锁群 C2 上，分别解释了表型变异的 45.21%和 25.97%。发现了四个与 EPE 相关的 QTL（MLG H 上的 QEPE-1、MLG G 上的 QEPE-2 和 MLG G 上的 QEPE-3），分别解释了表型变异的 7.11%、7.56%和 6.12%。发现了一个与 ESE 相关的 QTL，QESE-1（Satt427），位于连锁群 G 上，解释了表型变异的 6.99%。QEPE-2 和 QESE-1 位于 MLG G 的相似区域。这些 QTL 为具有更高胚胎发生能力的基因型提供了标记辅助选择的潜力。
