Chen Jun, Kang Liang, Zhang Nian
Laboratory of Developmental Genetics, Van Andel Research Institute, Grand Rapids, Michigan 49503, USA.
Genesis. 2005 Dec;43(4):196-204. doi: 10.1002/gene.20171.
Recent studies show that the cyclic expression of Lfng in the presomitic mesoderm (PSM) is controlled at the transcription level by Notch signal through the CBF1 binding site for activation and periodic repression on the Lfng promoter. Here we provide genetic evidence that the oscillatory expression is controlled by a negative feedback mechanism. We also show that Hes7, another cyclically expressed protein, can bind to the N-boxes on both Lfng and its own promoters and repress their activity. In addition, we demonstrate that the 3' untranslated region (3'-UTR) is important for rapid degradation of Lfng mRNA.
最近的研究表明，在体节发生前中胚层（PSM）中Lfng的周期性表达在转录水平上由Notch信号通过CBF1结合位点控制，以激活Lfng启动子并对其进行周期性抑制。在此，我们提供了遗传证据，证明这种振荡表达受负反馈机制控制。我们还表明，另一种周期性表达的蛋白质Hes7可以结合Lfng及其自身启动子上的N盒并抑制它们的活性。此外，我们证明3'非翻译区（3'-UTR）对Lfng mRNA的快速降解很重要。
