Yang C M, Cousins S W
Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Florida.
Invest Ophthalmol Vis Sci. 1992 Jul;33(8):2436-42.
This report postulates that the activity of cellular proliferation along the surface of the retina during proliferative vitreoretinopathy (PVR) is reflected in the aggregate effect of proliferation-inducing growth factors in the vitreous. A method for quantifying the "net" proliferation-inducing capacity of an individual vitreous sample was developed and this assay was used to evaluate the vitreous proliferative activity in a model for experimental PVR. Vitreous was aspirated sequentially after onset of fibroblast-induced PVR in a rabbit model. A simple bioassay for the "aggregate" stimulating activity was developed and each sample was assigned a quantitative value in terms of "proliferation units" (PU). Experimental eyes demonstrated a wide range of stimulating activity (0-1765 PU), but control eyes showed uniformly low levels of activity (0-337 PU). Experimental eyes that ultimately developed retinal detachment displayed higher levels of proliferative activity than did those eyes destined to remain attached. The differences were statistically different by day 3, prior to the onset of clinical retinal changes. We conclude that quantification of vitreous proliferation-stimulating activity is possible and that this method might be useful for screening eyes at high risk for the development of recurrent retinal detachment from PVR.
本报告假定，增殖性玻璃体视网膜病变（PVR）期间视网膜表面的细胞增殖活动反映在玻璃体内增殖诱导生长因子的总体作用中。开发了一种量化单个玻璃体样本“净”增殖诱导能力的方法，并使用该检测方法评估实验性PVR模型中的玻璃体增殖活性。在兔模型中，成纤维细胞诱导的PVR发作后依次抽取玻璃体。开发了一种针对“总体”刺激活性的简单生物检测方法，并根据“增殖单位”（PU）为每个样本赋予一个定量值。实验眼表现出广泛的刺激活性（0 - 1765 PU），但对照眼的活性水平均较低（0 - 337 PU）。最终发生视网膜脱离的实验眼比注定保持附着的眼表现出更高水平的增殖活性。在临床视网膜变化出现之前的第3天，差异具有统计学意义。我们得出结论，量化玻璃体增殖刺激活性是可行的，并且该方法可能有助于筛查有PVR复发导致视网膜脱离高风险的眼睛。
