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Appl Environ Microbiol. 1982 Dec;44(6):1385-8. doi: 10.1128/aem.44.6.1385-1388.1982.
2
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本文引用的文献

1
Effect of nitrate in the rooting medium on carbohydrate composition of soybean nodules.生根培养基中硝酸盐对大豆根瘤碳水化合物组成的影响。
Plant Physiol. 1981 Oct;68(4):840-4. doi: 10.1104/pp.68.4.840.
2
Induction of Root Nodule Senescence by Combined Nitrogen in Pisum sativum L.联合氮素诱导豌豆根瘤衰老
Plant Physiol. 1977 Mar;59(3):440-2. doi: 10.1104/pp.59.3.440.
3
Comparison of carbon monoxide, nitric oxide, and nitrite as inhibitors of the nitrogenase from Clostridium pasteurianum.一氧化碳、一氧化氮和亚硝酸盐作为巴氏梭菌固氮酶抑制剂的比较。
Arch Biochem Biophys. 1981 Aug;210(1):246-56. doi: 10.1016/0003-9861(81)90186-7.
4
Purification and characterization of the molybdenum-iron protein component of nitrogenase from soybean nodule bacteroids.大豆根瘤类菌体中固氮酶钼铁蛋白组分的纯化与特性分析
J Biol Chem. 1974 Jan 25;249(2):500-8.
5
Nitrate reductase from bacteroides of Rhizobium japonicum: enzyme characteristics and possible interaction with nitrogen fixation.来自日本根瘤菌拟杆菌的硝酸还原酶：酶的特性及与固氮作用的可能相互作用
Biochim Biophys Acta. 1975 Jul 27;397(1):24-35. doi: 10.1016/0005-2744(75)90175-8.
6
Interactions among substrates and inhibitors of nitrogenase.固氮酶的底物与抑制剂之间的相互作用。
J Bacteriol. 1975 Aug;123(2):537-45. doi: 10.1128/jb.123.2.537-545.1975.
7
The hydrogen cycle in nitrogen-fixing Azotobacter chroococcum.固氮圆褐固氮菌中的氢循环
Biochimie. 1978;60(3):225-31. doi: 10.1016/s0300-9084(78)80818-9.
8
Effect of nitrite upon leghemoglobin and interaction with nitrogen fixation.亚硝酸盐对豆血红蛋白的影响及其与固氮作用的相互关系
Biochim Biophys Acta. 1977 May 26;497(3):702-6. doi: 10.1016/0304-4165(77)90291-4.

亚硝酸盐和一氧化氮作为大豆类菌体固氮酶的抑制剂。

Nitrite and nitric oxide as inhibitors of nitrogenase from soybean bacteroids.

机构信息

Laboratoire de Biologie végétale, Faculté des Sciences et des Techniques, Parc Valrose, 06034 Nice Cedex, France.

出版信息

Appl Environ Microbiol. 1982 Dec;44(6):1385-8. doi: 10.1128/aem.44.6.1385-1388.1982.

DOI:10.1128/aem.44.6.1385-1388.1982
PMID:16346155
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC242200/
Abstract

Nitrite was able to strongly inhibit C(2)H(2) reduction by nitrogenase from soybean bacteroids, whereas H(2) evolution was unaffected under the same conditions. NO inhibited both C(2)H(2) reduction and H(2) evolution; during C(2)H(2) reduction, sensitivity of nitrogenase to NO was higher than to NO(2), and the K(i) values were, respectively, 0.056 and 0.52 mM. Production of NO resulting from a reduction of NO(2) by dithionite in nitrogenase incubations was observed. However, the characteristics of inhibitions and the low level of NO generated by nitrite reduction ruled out the suggestion concerning a direct role of NO to explain the inhibitory effect of NO(2) on nitrogenase.

摘要

亚硝酸盐能够强烈抑制大豆类菌体固氮酶催化的乙炔还原，而相同条件下氢气的释放则不受影响。一氧化氮（NO）同时抑制乙炔还原和氢气的释放；在乙炔还原过程中，固氮酶对 NO 的敏感性高于对亚硝酸盐（NO2），其 Ki 值分别为 0.056 和 0.52mM。在固氮酶孵育过程中，观察到二硫代苏糖醇将亚硝酸盐还原生成一氧化氮（NO）。然而，亚硝酸盐还原产生的一氧化氮抑制作用的特点和低水平生成，排除了关于一氧化氮（NO）直接发挥作用来解释亚硝酸盐（NO2）对固氮酶抑制的假说。