罗沙米星的生产：合成培养基的改良。
Production of rosamicin: improvement of synthetic medium.
机构信息
The Korea Advanced Institute of Science, Chung-Ryang-Ri, Seoul, Korea.
出版信息
Appl Environ Microbiol. 1983 Jan;45(1):350-3. doi: 10.1128/aem.45.1.350-353.1983.
Rosamicin is one of the important macrolide antibiotics that has clinical efficacy and broad-spectrum antibacterial activity. Using a mutant strain of Micromonospora rosaria (NRRL 3718), a chemically defined medium was developed, and some fermentation conditions that are important to rosamicin biosynthesis were optimized to achieve rosamicin productivity of 230 mug/ml. Soluble starch and l-asparagine were found to be the best carbon and nitrogen sources, and a stimulative effect of magnesium and zinc ions was also found. The medium developed contains: soluble starch, 4%; l-asparagine, 0.15%; K(2)HPO(4), 0.075%; CaCO(3), 0.6%; MgSO(4) . 7H(2)O, 0.05%; FeSO(4) . 7H(2)O, 10 M; CuSO(4) . 5H(2)O, 10 M; ZnSO(4) . 7H(2)O, 10 M; and MnSO(4) . (4-6)H(2)O, 10 M. The required air supply was about 40 mmol of O(2) liter . h . atm, and the favorable culture temperature was 28 to 29 degrees C.
罗沙米星是具有临床疗效和广谱抗菌活性的重要大环内酯类抗生素之一。利用玫瑰孢链霉菌（NRRL 3718）的突变株，开发了一种化学成分确定的培养基，并对一些对罗沙米星生物合成很重要的发酵条件进行了优化，使罗沙米星的产量达到 230 微克/毫升。发现可溶性淀粉和 L-天冬酰胺是最佳的碳源和氮源，还发现镁和锌离子有刺激作用。所开发的培养基含有：可溶性淀粉，4%；L-天冬酰胺，0.15%；K 2 HPO 4 ，0.075%；CaCO 3 ，0.6%；MgSO 4. 7H 2 O，0.05%；FeSO 4. 7H 2 O，10 M；CuSO 4. 5H 2 O，10 M；ZnSO 4. 7H 2 O，10 M；以及 MnSO 4. (4-6)H 2 O，10 M。所需的供气量约为 40 mmol O 2 升. h. atm，最适培养温度为 28 至 29 摄氏度。
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