改良的双瓶放射性呼吸计量技术用于 C 标记底物的矿化。
Improved double-vial radiorespirometric technique for mineralization of C-labeled substrates.
机构信息
W. K. Kellogg Biological Station, Michigan State University, Hickory Corners, Michigan 49060.
出版信息
Appl Environ Microbiol. 1984 May;47(5):1154-7. doi: 10.1128/aem.47.5.1154-1157.1984.
Abstract
New procedures which simplify sample preparation and improve counting efficiency were developed for double-vial radiorespirometry. Under certain conditions, efficiency of fluor-NaOH-impregnated wicks was not stable, but was adversely affected by water vapor, NaOH concentration, and CO(2) loading. Glass fiber wick material treated with a methanolic fluor-NaOH solution showed improved C counting efficiency (58%) compared with previous methods.
摘要
新的程序简化了样品准备并提高了计数效率，用于双瓶放射性呼吸测量。在某些条件下，氟-氢氧化钠浸渍的吸液芯的效率不稳定，而是受到水蒸气、氢氧化钠浓度和 CO(2)负载的不利影响。用甲醇氟-氢氧化钠溶液处理的玻璃纤维吸液芯材料与以前的方法相比，提高了 C 计数效率（58%）。
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