Wiig H, Sibley L, DeCarlo M, Renkin E M
Department of Human Physiology, School of Medicine, University of California, Davis 95616.
Am J Physiol. 1991 Jul;261(1 Pt 2):H155-65. doi: 10.1152/ajpheart.1991.261.1.H155.
A modification of the implanted wick method (K. Aukland and H. O. Fadnes. Acta Physiol. Scand. 88: 350-358, 1973) was devised to sample interstitial fluid from rat muscles. Dry nylon wicks were inserted postmortem into intermuscular spaces between leg muscles by means of a plastic catheter, which was subsequently withdrawn. Inserting the wicks postmortem avoids contaminating wick fluid with proteins extravasated as a result of local inflammatory reactions; placing them intermuscularly avoids contamination by fluid and proteins from damaged muscle cells. Wick fluid protein concentrations (mg/ml) averaged 24.1 +/- 1.1 and 28.5 +/- 1.5 (means +/- SE) in medial and lateral hindlimbs muscles, respectively. The corresponding albumin concentrations were 13.0 +/- 0.7 and 13.9 +/- 0.7 mg/ml. Total protein and albumin concentrations in plasma were 54.1 +/- 0.8 and 22.5 +/- 0.3 mg/ml. Electrophoresis of wick fluid showed a pattern of peaks similar to that of plasma, with albumin relatively high and larger molecules relatively low. Proteins from muscle cells were not detected. Isotope studies (125I-labeled albumin, 51Cr-EDTA) showed that less than 2% of the albumin in wick fluid came directly from plasma and that wick fluid was not concentrated by cell swelling postmortem. Wick fluid from intermuscular wicks implanted in anesthetized rats in vivo had nearly the same total protein concentration as fluid from postmortem wicks, but albumin-to-globulin (A/G) ratios were slightly lower (1.22 +/- 0.07 vs. 1.53 +/- 0.21 measured by gel electrophoresis), and more significantly, nearly 50% of the albumin leaked to wick fluid from plasma as a result of wick implantation.(ABSTRACT TRUNCATED AT 250 WORDS)
设计了一种改良的植入灯芯法（K. 奥克兰德和H. O. 法德内斯。《生理学杂志》斯堪的纳维亚版88: 350 - 358, 1973），用于从大鼠肌肉中采集间质液。死后通过塑料导管将干燥的尼龙灯芯插入腿部肌肉之间的肌间隙，随后拔出导管。死后插入灯芯可避免灯芯液被因局部炎症反应而渗出的蛋白质污染；将灯芯置于肌间可避免被受损肌肉细胞的液体和蛋白质污染。内侧和外侧后肢肌肉中灯芯液蛋白质浓度（mg/ml）分别平均为24.1±1.1和28.5±1.5（均值±标准误）。相应的白蛋白浓度分别为13.0±0.7和13.9±0.7 mg/ml。血浆中总蛋白和白蛋白浓度分别为54.1±0.8和22.5±0.3 mg/ml。灯芯液的电泳显示出与血浆相似的峰型模式，白蛋白相对较高，大分子相对较低。未检测到来自肌肉细胞的蛋白质。同位素研究（125I标记白蛋白、51Cr - 乙二胺四乙酸）表明，灯芯液中白蛋白不到2%直接来自血浆，且死后灯芯液不会因细胞肿胀而浓缩。在体内麻醉大鼠中植入肌间灯芯所获得的灯芯液，其总蛋白浓度与死后灯芯液几乎相同，但白蛋白与球蛋白（A/G）比值略低（凝胶电泳测得分别为1.22±0.07和1.53±0.21），更显著的是，由于灯芯植入，近50%的白蛋白从血浆漏入灯芯液。（摘要截短于250字）
