School of Engineering and Department of Microbiology, University of Guelph. Guelph. Ontario. NIG 2W1, Canada.
Appl Environ Microbiol. 1984 Sep;48(3):633-8. doi: 10.1128/aem.48.3.633-638.1984.
In a downflow stationary fixed-film anaerobic reactor receiving a swine waste influent, few bacteria were observed to be tightly adherent to the surfaces of the needle-punched polyester support material. However, there was a morphologically complex, dense population of bacteria trapped within the matrix. Frequently large microcolonies of a uniform morphological type of bacteria were observed. These were particularly evident for methanosarcina-like bacteria which grew forming large aggregates of unseparated cells. Leafy deposits of electron-dense, calcium- and phosphorus-enriched material coated the polyester matrix and some cells. As the biofilm matured there was more extensive mineral deposition which completely entrapped cells. The entrapped cells appeared to autolyze, and many were partially degraded. Further impregnation of the matrix with minerals and apparent cell death may eventually have a deleterious effect on the methanogenic activity of the biofilm.
在一个接收猪粪废水的下行式固定床厌氧反应器中，观察到很少有细菌紧密附着在针刺聚酯支撑材料的表面。然而，在基质中有形态复杂、密集的细菌群体被困住。经常可以观察到形态类型一致的大型微生物菌落。对于类似于产甲烷菌的细菌来说，这种情况尤其明显，它们形成了未分离细胞的大聚集。叶子状的电子致密、富含钙和磷的物质覆盖在聚酯基质和一些细胞上。随着生物膜的成熟，有更多的矿物质沉积，完全困住了细胞。被困住的细胞似乎自溶，许多细胞部分降解。基质中矿物质的进一步渗透和明显的细胞死亡最终可能对生物膜的产甲烷活性产生有害影响。
